Levels of Neurofilament Light at the Preataxic and Ataxic Stages of Spinocerebellar Ataxia Type 1.

Abstract

BACKGROUND AND OBJECTIVES:

Neurofilament light (NfL) appears to be a promising fluid biomarker in repeat-expansion spinocerebellar ataxias (SCAs), with piloting studies in mixed SCA cohorts suggesting that NfL might be increased at the ataxic stage of SCA type 1 (SCA1). We here hypothesized that NfL is increased not only at the ataxic stage of SCA1, but also at its (likely most treatment-relevant) preataxic stage.

METHODS:

We assessed serum NfL (sNfL) and CSF NfL (cNfL) levels in both preataxic and ataxic SCA1, leveraging a multicentric cohort recruited at 6 European university centers, and clinical follow-up data, including actually observed (rather than only predicted) conversion to the ataxic stage. Levels of sNfL and cNfL were assessed by single-molecule array and ELISA technique, respectively.

RESULTS:

Forty individuals with SCA1 (23 preataxic, 17 ataxic) and 89 controls were enrolled, including 11 preataxic individuals converting to the ataxic stage. sNfL levels were increased at the preataxic (median 15.5 pg/mL [interquartile range 10.5-21.1 pg/mL]) and ataxic stage (31.6 pg/mL [26.2-37.7 pg/mL]) compared to controls (6.0 pg/mL [4.7-8.6 pg/mL]), yielding high age-corrected effect sizes (preataxic: r = 0.62, ataxic: r = 0.63). sNfL increases were paralleled by increases of cNfL at both the preataxic and ataxic stage. In preataxic individuals, sNfL levels increased with proximity to predicted ataxia onset, with significant sNfL elevations already 5 years before onset, and confirmed in preataxic individuals with actually observed ataxia onset. sNfL increases were detected already in preataxic individuals with SCA1 without volumetric atrophy of cerebellum or pons, suggesting that sNfL might be more sensitive to early preataxic neurodegeneration than the currently known most change-sensitive regions in volumetric MRI. Using longitudinal sNfL measurements, we estimated sample sizes for clinical trials with the reduction of sNfL as the endpoint.

DISCUSSION:

sNfL levels might provide easily accessible peripheral biomarkers in both preataxic and ataxic SCA1, allowing stratification of preataxic individuals regarding proximity to onset, early detection of neurodegeneration even before volumetric MRI alterations, and potentially capture of treatment response in clinical trials.

TRIAL REGISTRATION INFORMATION:

ClinicalTrials.gov Identifier: NCT01037777.

CLASSIFICATION OF EVIDENCE:

This study provides Class III evidence that NfL levels are increased in both ataxic and preataxic SCA1 and are associated with ataxia onset.

• Authors: Carlo Wilke, David Mengel, Ludger Schöls, Holger Hengel, Maria Rakowicz, Thomas Klockgether, Alexandra Durr, Alessandro Filla, Bela Melegh, Rebecca Schüle, Kathrin Reetz, Heike Jacobi, Matthis Synofzik
• Journal: Neurology (Neurology) Vol. 98 Issue 20 Pg. e1985-e1996 (05 17 2022) ISSN: 1526-632X [Electronic] United States
• PMID: 35264424 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 American Academy of Neurology.

Chemical References

• Biomarkers
• Neurofilament Proteins

Topics

• Atrophy (pathology)
• Biomarkers
• Cerebellar Ataxia (pathology)
• Cerebellum (pathology)
• Humans
• Intermediate Filaments
• Neurofilament Proteins
• Spinocerebellar Ataxias (diagnosis)