A Novel Pneumococcal Surface Protein K of Nonencapsulated Streptococcus pneumoniae Promotes Transmission among Littermates in an Infant Mouse Model with Influenza A Virus Coinfection.

Abstract	We established an infant mouse model for colonization and transmission by nonencapsulated Streptococcus pneumoniae (NESp) strains to gain important information about its virulence among children. Invasive pneumococcal diseases have decreased dramatically since the worldwide introduction of pneumococcal capsular polysaccharide vaccines. Increasing prevalence of nonvaccine serotypes, including NESp, has been highlighted as a challenge in treatment strategy, but the virulence of NESp is not well understood. Protective strategies against NESp colonization and transmission between children require particularly urgent evaluation. NESp lacks capsules, a major virulence factor of pneumococci, but can cause a variety of infections in children and older people. PspK, a specific surface protein of NESp, is a key factor in establishing nasal colonization. In our infant mouse model for colonization and transmission by NESp strains, NESp could establish stable nasal colonization at the same level as encapsulated serotype 6A in infant mice and could be transmitted between littermates. Transmission was promoted by NESp surface virulence factor PspK and influenza virus coinfection. However, PspK deletion mutants lost the ability to colonize and transmit to new hosts. Promotion of NESp transmission by influenza was due to increased susceptibility of the new hosts. PspK was a key factor not only in establishment of nasal colonization but also in transmission to new hosts. PspK may be targeted as a new candidate vaccine for NESp infection in children.
Authors	Hideki Sakatani, Masamitsu Kono, Denisa Nanushaj, Daichi Murakami, Saori Takeda, Fumie Kaneko, Moon H Nahm, Muneki Hotomi
Journal	Infection and immunity (Infect Immun) Vol. 90 Issue 2 Pg. e0062221 (02 17 2022) ISSN: 1098-5522 [Electronic] United States
PMID	34978928 (Publication Type: Journal Article)
Chemical References	Pneumococcal Vaccines Virulence Factors Darbepoetin alfa
Topics	Aged Animals Coinfection Darbepoetin alfa (metabolism) Disease Models, Animal Humans Influenza A virus (genetics) Mice Pneumococcal Infections Pneumococcal Vaccines Streptococcus pneumoniae Virulence Factors (genetics, metabolism)

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