Given the role of macrophage-derived high mobility group box 1 (
HMGB1) in
chemotherapy-induced
peripheral neuropathy (CIPN) caused by
paclitaxel, we analyzed the role of
HMGB1 and macrophages in the CIPN caused by
bortezomib, a
proteasome-inhibiting chemotherapeutic agent used for the treatment of
multiple myeloma. Repeated administration of
bortezomib caused CIPN accompanied by early-stage macrophage accumulation in the dorsal root ganglion. This CIPN was prevented by an anti-HMGB1-neutralizing antibody,
thrombomodulin alfa capable of accelerating
thrombin-dependent degradation of
HMGB1, antagonists of the
receptor for advanced glycation end-products (RAGE) and C-X-C motif
chemokine receptor 4 (CXCR4), known as HMGB1-targeted membrane receptors, or macrophage depletion with liposomal
clodronate, as reported in a CIPN model caused by
paclitaxel. In macrophage-like RAW264.7 cells,
bortezomib as well as
MG132, a well-known
proteasome inhibitor, caused
HMGB1 release, an effect inhibited by
caspase inhibitors but not inhibitors of NF-κB and
p38 MAP kinase, known to mediate
paclitaxel-induced
HMGB1 release from macrophages.
Bortezomib increased cleaved products of
caspase-8 and caused nuclear fragmentation or condensation in macrophages. Repeated treatment with the
caspase inhibitor prevented CIPN caused by
bortezomib in mice. Our findings suggest that
bortezomib causes
caspase-dependent release of
HMGB1 from macrophages, leading to the development of CIPN via activation of RAGE and CXCR4.