ssc-microRNA-132 targets DACH1 to exert anti-inflammatory and anti-apoptotic effects in Clostridium perfringens beta2 toxin-treated porcine intestinal epithelial cells.

Abstract	Clostridium perfringens (C. perfringens) type C (CPC) is one of the chief pathogens that causes diarrhea in piglets, and C. perfringens beta2 (CPB2) toxin is the main virulence factor of CPC. Our previous research demonstrated that ssc-microR-132 was differentially expressed in ileal tissues of CPC-mediated diarrheic piglets and healthy piglets, which implied a potential role of ssc-microR-132 in this process. Here, we found that ssc-microR-132 was notably down-regulated in CPB2-exposed intestinal porcine epithelial cells (IPEC-J2), which was consistent with the ileal tissue expression. Moreover, ssc-microR-132 upregulation alleviated CPB2-induced inflammatory damage and apoptosis in IPEC-J2, whereas ssc-microR-132 knockdown presented the opposite effects. Furthermore, the dual-luciferase reporter assay indicated that ssc-microR-132 directly targeted Dachshund homolog 1 (DACH1). Moreover, DACH1 overexpression intensified CPB2-induced inflammatory injury and apoptosis in IPEC-J2. Remarkably, the introduction of DACH1 weakened the anti-inflammatory and anti-apoptotic effects of ssc-microR-132 in CPB2-exposed IPEC-J2. Overall, the results reveal that ssc-microR-132 targeted DACH1 to alleviate CPB2-mediated inflammation and apoptosis in IPEC-J2.
Authors	Kaihui Xie, Zunqiang Yan, Wei Wang, Ruirui Luo, Xiaoli Gao, Pengfei Wang, Qiaoli Yang, Xiaoyu Huang, Juanli Zhang, Jiaojiao Yang, Shuangbao Gun
Journal	Developmental and comparative immunology (Dev Comp Immunol) Vol. 127 Pg. 104270 (02 2022) ISSN: 1879-0089 [Electronic] United States
PMID	34582881 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2021 Elsevier Ltd. All rights reserved.
Chemical References	Anti-Inflammatory Agents MicroRNAs
Topics	Animals Anti-Inflammatory Agents (metabolism) Cell Line Clostridium perfringens (genetics, metabolism) Epithelial Cells (metabolism) MicroRNAs (genetics, metabolism) Swine

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