Detection of Novel Urine Markers Using Immune Complexome Analysis in Bladder Cancer Patients: A Preliminary Study.

Abstract

BACKGROUND/AIM:

Little is known on urine biomarkers that are associated with malignant behavior in patients with bladder cancer (BC). Our aim was to identify BC-related factors in urine samples using our original method "immune complexome analysis", based on detecting the immune complex (IC).

PATIENTS AND METHODS:

Immune complexome analysis was performed using urine samples from 97 BC patients, including 67 with non-muscle invasive BC (NMIBC).

RESULTS:

Eight IC-antigens were recognized as candidates for BC-related factors from 20,165 proteins. IC-serum albumin, -fibrinogen γ chain, -hemoglobin subunit α, -hemoglobin subunit β, -ceruloplasmin, and fibrinogen β chain were significantly associated with either pathological features and/or outcome. IC-ceruloplasmin was most widely associated with pathological features in all BC patients and lamina propria invasion and urinary tract recurrence in NMIBC.

CONCLUSION:

Based on detection of IC-antigens it was demonstrated that six IC-antigens, especially IC-ceruloplasmin, are potential urine biomarkers in BC.

• Authors: Nozomi Aibara, Yasuyoshi Miyata, Kyohei Araki, Yuji Sagara, Kensuke Mitsunari, Tomohiro Matsuo, Kojiro Ohba, Yasushi Mochizuki, Hideki Sakai, Kaname Ohyama
• Journal: In vivo (Athens, Greece) (In Vivo) 2021 Jul-Aug Vol. 35 Issue 4 Pg. 2073-2080 ISSN: 1791-7549 [Electronic] Greece
• PMID: 34182482 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Biomarkers, Tumor

Topics

• Biomarkers, Tumor
• Humans
• Neoplasm Recurrence, Local
• Urinary Bladder Neoplasms (diagnosis)