Antiosteoarthritic Effect of Morroniside in Chondrocyte Inflammation and Destabilization of Medial Meniscus-Induced Mouse Model.

Abstract

Osteoarthritis (OA) is a common degenerative disease that results in joint inflammation as well as pain and stiffness. A previous study has reported that Cornus officinalis (CO) extract inhibits oxidant activities and oxidative stress in RAW 264.7 cells. In the present study, we isolated bioactive compound(s) by fractionating the CO extract to elucidate its antiosteoarthritic effects. A single bioactive component, morroniside, was identified as a potential candidate. The CO extract and morroniside exhibited antiosteoarthritic effects by downregulating factors associated with cartilage degradation, including cyclooxygenase-2 (Cox-2), matrix metalloproteinase 3 (Mmp-3), and matrix metalloproteinase 13 (Mmp-13), in interleukin-1 beta (IL-1β)-induced chondrocytes. Furthermore, morroniside prevented prostaglandin E2 (PGE2) and collagenase secretion in IL-1β-induced chondrocytes. In the destabilization of the medial meniscus (DMM)-induced mouse osteoarthritic model, morroniside administration attenuated cartilage destruction by decreasing expression of inflammatory mediators, such as Cox-2, Mmp3, and Mmp13, in the articular cartilage. Transverse microcomputed tomography analysis revealed that morroniside reduced DMM-induced sclerosis in the subchondral bone plate. These findings suggest that morroniside may be a potential protective bioactive compound against OA pathogenesis.

• Authors: Eunkuk Park, Chang Gun Lee, Seong Jae Han, Seung Hee Yun, Seokjin Hwang, Hyoju Jeon, Jeonghyun Kim, Chun Whan Choi, Siyoung Yang, Seon-Yong Jeong
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 6 (Mar 15 2021) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 33804203 (Publication Type: Journal Article)

Chemical References

• Glycosides
• IL1B protein, mouse
• Interleukin-1beta
• Plant Extracts
• morroniside
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• Matrix Metalloproteinase 13
• Matrix Metalloproteinase 3
• Dinoprostone

Topics

• Animals
• Cartilage, Articular (drug effects)
• Chondrocytes (drug effects)
• Cornus (chemistry)
• Cyclooxygenase 2 (genetics)
• Dinoprostone (genetics)
• Disease Models, Animal
• Gene Expression Regulation (drug effects)
• Glycosides (chemistry, pharmacology)
• Humans
• Inflammation (drug therapy)
• Interleukin-1beta (genetics)
• Matrix Metalloproteinase 13 (genetics)
• Matrix Metalloproteinase 3 (genetics)
• Menisci, Tibial (drug effects, pathology, surgery)
• Mice
• Osteoarthritis (drug therapy, genetics, pathology, surgery)
• Plant Extracts (chemistry, pharmacology)
• Primary Cell Culture
• RAW 264.7 Cells
• Signal Transduction (drug effects)