Abstract | OBJECTIVE: MATERIALS AND METHODS: Five groups of Wistar rats were used, and the groups included sham surgery ( Sham group), BDL with no treatment (NT group), BDL plus ICKT (ICKT group), BDL plus SRT (SRT group), and BDL plus ICKT and SRT (SRT/ICKT group). Each herbal medicine was administered at 1 g/kg/day on the first postoperative day. The serum levels and various clinical markers were measured with real-time polymerase chain reaction. Staining was used to evaluate the degree of fibrosis and the inflammatory responses. RESULTS: Serum aspartate aminotransferase and alanine aminotransferase in the ICKT and SRT/ICKT groups were significantly lower than those in the NT group. NF-κB mRNA expression was significantly decreased in the ICKT group and the SRT/ICKT group compared with the NT group. AQP9 mRNA expression was significantly increased in the ICKT group and the SRT/ICKT group compared with the NT group. The degree of Masson's trichrome staining in the SRT/ICKT group was significantly lower than that in the NT group. The degree of NF-κB staining in the SRT/ICKT group was significantly lower than that in the NT, ICKT, or SRT group. CONCLUSIONS: The postoperative administration of ICKT and SRT induced synergistic beneficial effects, resulting in the reduction of hepatic fibrosis via mechanisms involving the inhibition of NF-κB expression and the improvement of AQP9 downregulation.
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Authors | Naoki Hashizume, Ryusuke Shin, Jun Akiba, Naoki Sotogaku, Kimio Asagiri, Shigeki Hikida, Suguru Fukahori, Shinji Ishii, Nobuyuki Saikusa, Yoshinori Koga, Hideaki Egami, Yoshiaki Tanaka, Akinori Nishi, Minoru Yagi |
Journal | Pediatric surgery international
(Pediatr Surg Int)
Vol. 37
Issue 8
Pg. 1079-1088
(Aug 2021)
ISSN: 1437-9813 [Electronic] Germany |
PMID | 33710364
(Publication Type: Journal Article)
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Chemical References |
- Aqp9 protein, rat
- Aquaporins
- Drugs, Chinese Herbal
- NF-kappa B
- inchinko-to
- sairei-to
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Topics |
- Animals
- Aquaporins
(metabolism, pharmacology)
- Bile Ducts
(surgery)
- Cholestasis
(drug therapy)
- Disease Models, Animal
- Drugs, Chinese Herbal
(administration & dosage)
- Hepatocytes
(metabolism)
- Ligation
- Liver Cirrhosis
(metabolism)
- Male
- NF-kappa B
(metabolism)
- Rats
- Rats, Wistar
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