Evaluation of active matrix metalloproteinase-8 (aMMP-8) chair-side test as a diagnostic biomarker in the staging of periodontal diseases.

Abstract

OBJECTIVE:

There is a need for a reliable complementary diagnostic tool that ideally helps to screen, differentiate sites, activities of and predict future periodontal tissue destruction. The purpose of this cross-sectional study was to investigate the screening and prevention potential of the chair-side/point-of-care (PoC) diagnostic test of salivary active matrix metalloproteinase-8 (aMMP-8) levels at different stages of periodontal disease and periodontal health.

MATERIAL & METHODS:

80 individuals were included in this study; 18 with periodontitis stage 3 (P-Stage III), 19 with periodontitis stage-4 (P-Stage IV), 21 with gingivitis, and 22 with clinically healthy periodontium (H). The aMMP-8 levels in GCF and saliva were analyzed by chairside point-of-care aMMP-8 lateral flow immunotest and also by a time-resolved immunofluorescence assay (IFMA).

RESULTS:

The sensitivity of the chair-side/PoC test was 83.9 % while specificity was 79.2 %. The aMMP-8 IFMA levels in GCF were significantly higher in P-Stage IV group than P-Stage III, gingivitis and healthy groups (p = 0.01, p = 0.001, p = 0.00, respectively). Moreover, P-Stage III and gingivitis groups had significantly higher aMMP-8 IFMA levels than the healthy group (p < 0.05).

CONCLUSION:

The aMMP-8 chair-side test showed promising results in its ability to recognize and predict the inflammatory status even at the very initial/early stages. aMMP-8 chair-side test could be a valuable adjunctive diagnostic and preventive tool to conventional clinical methods in detecting periodontal disease.

• Authors: V Özgen Öztürk, Gülnur Emingil, Kehinde Umeizudike, Taina Tervahartiala, Dirk-Rolf Gieselmann, Kurt Maier, Timur Köse, Timo Sorsa, Saeed Alassiri
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 124 Pg. 104955 (Apr 2021) ISSN: 1879-1506 [Electronic] England
• PMID: 33556789 (Publication Type: Journal Article)
• Copyright: Copyright © 2020. Published by Elsevier Ltd.

Chemical References

• Biomarkers
• MMP8 protein, human
• Matrix Metalloproteinase 8

Topics

• Biomarkers (analysis)
• Cross-Sectional Studies
• Gingival Crevicular Fluid (chemistry)
• Gingivitis (diagnosis)
• Humans
• Matrix Metalloproteinase 8
• Periodontal Diseases (diagnosis)