Non-target metabolomic analysis reveals the therapeutic effect of Saposhnikovia divaricata decoction on collagen-induced arthritis rats.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Saposhnikovia divaricata (SD), a Chinese crude drug, has long been recognized for therapeutic effect to rheumatoid arthritis (RA). At present, the mechanisms of SD treatment in RA have not been fully understood especially on the perspective of metabolomics.

AIM OF THE STUDY:

To study the pharmacodynamic effects of Saposhnikovia divaricata decoction on CIA rats, and explore the therapeutic mechanism by metabolomics methods.

MATERIALS AND METHODS:

Wistar rats were randomly divided into normal group, CIA model group, dexamethasone group and SD decoction groups (10 g crude drug/kg, 5 g crude drug/kg and 2.5 g crude drug/kg of SDD). Body weight, arthritis scores, serum cytokine levels and histopathological parameters of rats were assessed. A metabolomics method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOFMS) was established to collect the metabolic profiles of rats and explore the metabolic changes that occurred after SDD treatment.

RESULTS:

SDD showed its protective effect on the affected joints, especially in the middle dosage group of SDD. Eighteen and 13 potential biomarkers for the SDD treatment of CIA rats were identified in the plasma and urine, respectively. SDD could regulate the disturbed metabolic pathways including tryptophan metabolism, glycerophospholipid catabolism, primary bile acid biosynthesis and fatty acid metabolism.

CONCLUSIONS:

In summary, SDD treatment could effectively alleviate symptoms of RA and regulate metabolic disorders in CIA rats.

• Authors: Yueyue Li, Diya Lv, Rong Liu, Yuhuan Shi, Rong Wang, Zhenyu Zhu, Yongfang Yuan
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 271 Pg. 113837 (May 10 2021) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 33460755 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Biomarkers
• Cytokines
• Drugs, Chinese Herbal
• Collagen

Topics

• Animals
• Ankle Joint (metabolism, pathology)
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Apiaceae (chemistry)
• Arthritis, Experimental (chemically induced, drug therapy, pathology)
• Biomarkers (blood, urine)
• Chromatography, High Pressure Liquid
• Collagen (toxicity)
• Cytokines (metabolism)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Male
• Mass Spectrometry
• Metabolome (drug effects)
• Metabolomics (methods)
• Rats, Wistar
• Severity of Illness Index
• Signal Transduction (drug effects)
• Rats