Abstract | BACKGROUND: METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of KRT16P3 in TSCC tissues and cells. Next, the relationships between KRT16P3 and the clinical significance of TSCC patients were analyzed. Additionally, Cell Counting Kit-8, 5-Bromo-2-deoxyuridine ( BrdU) incorporation assay, cell colony formation assay, flow cytometry cell apoptosis analysis, scratch wound healing assay, transwell invasion assay were used to explore the biological function of KRT16P3. Western blot and qRT-PCR were used to determine the expression of epithelial-mesenchymal transition (EMT) markers. The pathway changes after KRT16P3 knockdown were detected by Western blot. RESULTS: We found KRT16P3 expression is significantly upregulated in TSCC tissues and positively associated with advanced clinicopathological features of TSCC patients, and it may serve as a poor prognostic factor. Functionally, KRT16P3 knockdown inhibits proliferation, migration, invasion and promotes apoptosis of TSCC cells. Furthermore, we also revealed that KRT16P3 knockdown suppresses EMT and JAK2/STAT3 signaling pathway. CONCLUSION: Our results validated that KRT16P3 can modulate the malignant progression, EMT process, and JAK2/STAT3 signaling pathway of TSCC, which might also serve as a novel prognostic biomarker and an attractive target for TSCC patients.
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Authors | Yifan Yang, Ling Feng, Hongzhi Ma, Ru Wang, Jugao Fang |
Journal | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
(J Oral Pathol Med)
Vol. 50
Issue 4
Pg. 385-393
(Apr 2021)
ISSN: 1600-0714 [Electronic] Denmark |
PMID | 33220105
(Publication Type: Journal Article)
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Copyright | © 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
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Topics |
- Biomarkers
- Carcinoma, Squamous Cell
(genetics)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Epithelial-Mesenchymal Transition
(genetics)
- Gene Expression Regulation, Neoplastic
- Humans
- Prognosis
- Tongue
- Tongue Neoplasms
(genetics)
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