Abstract |
Patrinia villosa (Thunb.) Juss is a traditional herb commonly used in East Asia including Korea, Japan, and China. It has been administered to reduce and treat inflammation in Donguibogam, Korea. The mechanism for its anti-inflammatory effects has already been reported. In this study, we confirmed the efficacy of Patrinia villosa (Thunb.) Juss ethanol extract (Pv-EE) for inducing autophagy and investigate its anti-melanogenic properties. Melanin secretion and content were investigated using cells from the melanoma cell line B16F10. Pv-EE inhibited melanin in melanogenesis induced by α- melanocyte-stimulating hormone (α- MSH). The mechanism of inhibition of Pv-EE was confirmed by suppressing the mRNA of microphthalmia-associated transcription factor (MITF), decreasing the phosphorylation level of CREB, and increasing the phosphorylation of ERK. Finally, it was confirmed that Pv-EE induces autophagy through the autophagy markers LC3B and p62, and that the anti-melanogenic effect of Pv-EE is inhibited by the autophagy inhibitor 3-methyl adenine (3-MA). These results suggest that Pv-EE may be used as a skin protectant due to its anti- melanin properties including autophagy.
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Authors | Deok Jeong, Sang Hee Park, Min-Ha Kim, Sarah Lee, Yoon Kyung Cho, You Ah Kim, Byoung Jun Park, Jongsung Lee, Hakhee Kang, Jae Youl Cho |
Journal | Molecules (Basel, Switzerland)
(Molecules)
Vol. 25
Issue 22
(Nov 17 2020)
ISSN: 1420-3049 [Electronic] Switzerland |
PMID | 33212959
(Publication Type: Journal Article)
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Chemical References |
- Cyclic AMP Response Element-Binding Protein
- Melanins
- Microphthalmia-Associated Transcription Factor
- Plant Extracts
- RNA, Messenger
- Ethanol
- alpha-MSH
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Topics |
- Animals
- Autophagy
(drug effects)
- Cyclic AMP Response Element-Binding Protein
(metabolism)
- Ethanol
(chemistry)
- Gene Expression Regulation
(drug effects)
- MAP Kinase Signaling System
(drug effects)
- Melanins
(metabolism)
- Melanoma, Experimental
(pathology)
- Mice
- Microphthalmia-Associated Transcription Factor
(genetics, metabolism)
- Models, Biological
- Patrinia
(chemistry)
- Plant Extracts
(pharmacology)
- Plant Leaves
(chemistry)
- Plant Roots
(chemistry)
- RNA, Messenger
(genetics, metabolism)
- alpha-MSH
(pharmacology)
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