Aqueous humor (AH)
proteins are involved in many physiological and
pathological processes of the eye. The
proteome analysis of AH is important to understand its physiological and pathophysiological functions. In the present study, AH samples obtained from 21
cataract volunteers were pooled together. After high-pH RPLC offline separation, the pooled sample was analyzed by LC-MS/MS to provide a comprehensive profile of AH
proteome. The function analysis was provided by the GO and IPA annotation. In order to determine whether the AH
proteome can reflect the pathophysiological changes of the disease,
DIA technology was used to analyze the AH samples obtained from three
neovascular glaucoma (NVG) patients (six samples) before and after drug treatment. The differential
proteins were validated by PRM technology in an independent group (14 samples). In the AH
proteome database, 802
proteins were identified, and 318
proteins were identified for the first time. Furthermore, 480
proteins were quantified based on the peak intensity-based semiquantification (iBAQ), which ranged by approximately 7 orders of magnitude. These
proteins are primarily involved in immunity- and
inflammation-related pathways. The differential AH proteomic analysis in NVG treatment revealed that the AH
proteome can reflect the pathophysiological changes of drug treatment. Angiogenesis and
thrombus coagulation progression are deeply involved in NVG treatment. The present experiment provided a comprehensive AH
proteome analysis and expanded the profile of human AH
proteome. The differential AH proteomic analysis of NVG treatment indicated that AH
proteome can reflect the pathophysiological changes in drug intervention.