Abstract | BACKGROUND: MATERIALS/METHODS: Male Fischer-344 rats were treated with l- Nicotine (1.89 mg/kg/day s.c., N = 10) or NaCl solution (N = 10). After a week of premedication, a NiTi spring was inserted to mesialize the first upper left molar. The extent of dental root resorption, osteoclastogenesis, and HIF-1α protein expression was determined by (immuno)histology, as well as bone volume (BV/TV) and trabecular thickness (TbTh) using µCT. Receptor activator of nuclear factor of activated B-cells ligand (RANK-L), osteoprotegerin (OPG), and HIF-1α expression were examined at the protein level in periodontal ligament fibroblasts (PDLF) exposed to pressure, nicotine and/or hypoxia, as well as PDLF-induced osteoclastogenesis in co-culture experiments with osteoclast progenitor cells. RESULTS:
Nicotine favoured dental root resorptions and osteoclastogenesis during OTM, while BV/TV and TbTh were only influenced by force. This nicotine-induced increase does not appear to be mediated by HIF-1α, since HIF-1α was stabilized by force application and hypoxia, but not by nicotine. The in vitro data showed that the hypoxia-induced increase in RANK-L/OPG expression ratio and PDLF-mediated osteoclastogenesis was less pronounced than the nicotine-induced increase. CONCLUSIONS: Study results indicate that the nicotine-induced increase in osteoclastogenesis and periodontal bone resorption during OTM may not be mediated by hypoxic effects or HIF-1α stabilization in the context of nicotine-induced vasoconstriction, but rather by an alternative mechanism.
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Authors | Niklas Ullrich, Agnes Schröder, Maria Bauer, Gerrit Spanier, Jonathan Jantsch, James Deschner, Peter Proff, Christian Kirschneck |
Journal | European journal of orthodontics
(Eur J Orthod)
Vol. 43
Issue 5
Pg. 516-526
(10 04 2021)
ISSN: 1460-2210 [Electronic] England |
PMID | 33043973
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author(s) 2020. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: [email protected]. |
Chemical References |
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Topics |
- Alveolar Bone Loss
- Animals
- Bone Resorption
(chemically induced)
- Male
- Nicotine
(toxicity)
- Osteoclasts
- Periodontal Ligament
- RANK Ligand
- Rats
- Root Resorption
(etiology)
- Tooth Movement Techniques
(adverse effects)
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