Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation.

Abstract

Background: In humans, resistance to thyroid hormone (RTH) caused by mutations in the thyroid hormone receptor alpha (THRA) gene, RTHα, manifests as tissue-specific hypothyroidism and circulating thyroid hormone levels exhibit hypothyroid-like clinical features. Before the identification of patients with RTHα, several Thrα1 knock-in mouse models were generated to clarify the function of TRα1. However, the phenotypes of these mice were not consistent with the clinical presentation of RTHα in humans. For the present study, we generated an RTHα mouse model that carries the Thra1E403X mutation found in human RTHα patients. Here, we report the gross phenotypes of this mouse RTHα model. Methods: Traditional homologous recombination gene targeting techniques were used to introduce a mutation (Thra1E403X) in the mouse Thra gene. The phenotypes of the resulting mice were studied and compared with clinical features observed for RTHα with THRAE403X. Results: Thrα1E403X/E403X homozygous mice exhibited severe neurological phenotypes, such as spasticity and motor ataxia, which were similar to those observed in endemic cretinism. Thrα1E403X/+ heterozygous mice reproduced most clinical manifestations of patient with RTHα, such as a normal survival rate and male fertility, as well as delayed postnatal growth and development, neurological and motor coordination deficits, and anemia. The mice had typical thyroid function with a modest increase in serum triiodothyronine (T3) levels, a low thyroxine (T4)/T3 ratio, and low reverse T3 (rT3) levels. Conclusions: The Thrα1E403X/+ mice faithfully recapitulate the clinical features of human RTHα and thus can provide a useful tool to dissect the role of TRα1 in development and to determine the pathological mechanisms of RTHα.

• Authors: Yue Liang, Defa Zhao, Ranran Wang, Pingping Dang, Yue Xi, Dan Zhang, Wei Wang, Zhongyan Shan, Xiaochun Teng, Weiping Teng
• Journal: Thyroid : official journal of the American Thyroid Association (Thyroid) Vol. 31 Issue 4 Pg. 678-691 (04 2021) ISSN: 1557-9077 [Electronic] United States
• PMID: 32924834 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Thyroid Hormone Receptors alpha
• Thyroid Hormones

Topics

• Animals
• Ataxia (genetics, metabolism, physiopathology)
• Bone Development
• Brain (growth & development)
• Disease Models, Animal
• Fertility
• Genetic Predisposition to Disease
• Heterozygote
• Homozygote
• Mice, Inbred C57BL
• Mice, Mutant Strains
• Motor Activity
• Muscle Spasticity (genetics, metabolism, physiopathology)
• Muscle Strength
• Mutation
• Phenotype
• Thyroid Gland (metabolism, physiopathology)
• Thyroid Hormone Receptors alpha (genetics, metabolism)
• Thyroid Hormone Resistance Syndrome (blood, genetics, physiopathology)
• Thyroid Hormones (blood)
• Mice