Abstract | OBJECTIVE: METHODS: The plasma samples were obtained from 112 sepsis patients within 24 hours after admission and 100 healthy controls (HCs) at enrollment. The lncRNA MEG3 expression in plasma samples was determined by RT-qPCR. In sepsis patients, ARDS occurrence was assessed based on Berlin definition of ARDS and 28-day mortality risk was evaluated. RESULTS:
LncRNA MEG3 expression was increased in sepsis patients compared with HCs. During 28-day duration, 30 sepsis patients occurred ARDS and 82 sepsis patients did not occur ARDS. LncRNA MEG3 expression was elevated in ARDS sepsis patients compared with non-ARDS sepsis patients, then the following receiver-operating characteristic (ROC) curve analysis disclosed that lncRNA MEG3 predicted ARDS risk (area under the curve (AUC) = 0.775), which was further validated as an independent risk factor by multivariate logistic regression. Furthermore, lncRNA MEG3 was positively correlated with chronic obstructive pulmonary disease, respiratory infection, acute physiology and chronic health evaluation II score, sequential organ failure assessment score, white blood cell, and C-reactive protein, while negatively correlated with albumin in sepsis patients. Additionally, lncRNA MEG3 was elevated in 28-day deaths compared with 28-day survivors, and it predicted 28-day mortality risk in sepsis patients (AUC = 0.708) by ROC curve analysis. CONCLUSION:
LncRNA MEG3 might represent as a valuable biomarker for individualizing prevention strategies against ARDS and improving prognosis in sepsis.
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Authors | Xiaoling Wu, Dan Chen, Li Yu |
Journal | Journal of clinical laboratory analysis
(J Clin Lab Anal)
Vol. 34
Issue 11
Pg. e23488
(Nov 2020)
ISSN: 1098-2825 [Electronic] United States |
PMID | 32844492
(Publication Type: Journal Article)
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Copyright | © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. |
Chemical References |
- MEG3 non-coding RNA, human
- RNA, Long Noncoding
|
Topics |
- Adult
- Aged
- Case-Control Studies
- Female
- Humans
- Male
- Middle Aged
- Prognosis
- RNA, Long Noncoding
(blood)
- Respiratory Distress Syndrome
(blood, diagnosis, epidemiology, etiology)
- Risk Factors
- Sensitivity and Specificity
- Sepsis
(blood, complications, diagnosis, epidemiology)
- Severity of Illness Index
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