Oral Administration of Sargassum horneri Improves the HDM/DNCB-Induced Atopic Dermatitis in NC/Nga Mice.

Abstract

The present study investigated the protective effects of Sargassum horneri (S. horneri) ethanol extract (SHE) against atopic dermatitis (AD), known as an abnormal immune response in house dust mite (HDM)/2,4-dinitrochlorobenzene (DNCB)-stimulated NC/Nga mice. The oral administration of SHE attenuated the AD symptoms, including the skin dermatitis severity, transepidermal water loss (TEWL), and ear edema in HDM/DNCB-stimulated mice. Moreover, the histological analysis revealed that SHE improved epidermal hyperplasia and hyperkeratosis, and reduced the dermal infiltrations of mast cells and eosinophils. Moreover, SHE downregulated the expression levels of cytokines (interleukin (IL)-6, IL-10, and interferon (IFN)-γ) and chemokines (Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES), Eotaxin, and Thymus and activation-regulated chemokine (TARC)) by decreasing the expression levels of atopic initiators (IL-25 and IL-33) in HDM/DNCB-stimulated skin. The oral administration of SHE decreased the spleen size, reducing expression levels of AD-related cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ, and TARC) by regulating the expressions of Tbx21 (T-bet), GATA Binding Protein 3 (GATA-3), and Signal transducer and activator of transcription 3 (STAT3). Moreover, SHE significantly attenuated the serum immunoglobulin (Ig)G1 and IgG2a levels in HDM/DNCB-stimulated mice. Collectively, these results suggest that S. horneri could be an ingredient of functional food against abnormal immune response.

• Authors: Eui Jeong Han, Ilekuttige Priyan Shanura Fernando, Hyun-Soo Kim, You-Jin Jeon, Dissanayaka Mudiyanselage Dinesh Madusanka, Mawalle Kankanamge Hasitha Madhawa Dias, Youngheun Jee, Ginnae Ahn
• Journal: Nutrients (Nutrients) Vol. 12 Issue 8 (Aug 18 2020) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 32824648 (Publication Type: Journal Article)

Chemical References

• Chemokines
• Cytokines
• Dinitrochlorobenzene
• GABA Plasma Membrane Transport Proteins
• Immunoglobulin G
• Plant Extracts
• STAT3 Transcription Factor
• Stat3 protein, mouse

Topics

• Administration, Oral
• Animals
• Chemokines (genetics, metabolism)
• Cytokines (genetics, metabolism)
• Dermatitis, Atopic (drug therapy, genetics, immunology, pathology)
• Dinitrochlorobenzene (immunology)
• Female
• Functional Food
• GABA Plasma Membrane Transport Proteins (metabolism)
• Gene Expression (drug effects)
• Immunoglobulin G (metabolism)
• Mice
• Plant Extracts (administration & dosage)
• Pyroglyphidae (immunology)
• STAT3 Transcription Factor (metabolism)
• Sargassum (chemistry)
• Severity of Illness Index