Anti-Photoaging and Anti-Melanogenesis Effects of Fucoidan Isolated from Hizikia fusiforme and Its Underlying Mechanisms.

Abstract

Previous studies suggested that fucoidan with a molecular weight of 102.67 kDa, isolated from Hizikia fusiforme, possesses strong antioxidant activity. To explore the cosmeceutical potential of fucoidan, its anti-photoaging and anti-melanogenesis effects were evaluated in the present study. The anti-photoaging effect was investigated in ultraviolet (UV) B-irradiated human keratinocytes (HaCaT cells), where fucoidan effectively reduced the intracellular reactive oxygen species level and improved the viability of the UVB-irradiated cells without any cytotoxic effects. Moreover, fucoidan significantly decreased UVB-induced apoptosis in HaCaT cells by regulating the protein expression of Bax, Bcl-xL, PARP, and Caspase-3 in HaCaT cells in a concentration-dependent manner. The anti-melanogenesis effect of fucoidan was evaluated in B16F10 melanoma cells that had been stimulated with alpha-melanocyte-stimulating hormone (α-MSH), and fucoidan treatment remarkably inhibited melanin synthesis in α-MSH-stimulated B16F10 cells. Further studies indicated that fucoidan significantly suppressed the expression of tyrosinase and tyrosinase-related protein-1 and -2 (TRP-1 and-2) in B16F10 cells by down-regulating microphthalmia-associated transcription factor (MITF) through regulation of the ERK-MAPK (extracellular signal regulated kinase-mitogen activated protein kinase) pathway. Taken together, these results suggest that fucoidan isolated from H. fusiforme possesses strong anti-photoaging and anti-melanogenesis activities and can be used as an ingredient in the pharmaceutical and cosmeceutical industries.

• Authors: Lei Wang, Jae-Young Oh, Young-Sang Kim, Hyo-Geun Lee, Jung-Suck Lee, You-Jin Jeon
• Journal: Marine drugs (Mar Drugs) Vol. 18 Issue 8 (Aug 15 2020) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 32824148 (Publication Type: Journal Article)

Chemical References

• Apoptosis Regulatory Proteins
• Dermatologic Agents
• Melanins
• Polysaccharides
• Reactive Oxygen Species
• fucoidan

Topics

• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Dermatologic Agents (isolation & purification, pharmacology)
• HaCaT Cells
• Humans
• Melanins (biosynthesis)
• Melanoma, Experimental
• Polysaccharides (isolation & purification, pharmacology)
• Reactive Oxygen Species (metabolism)
• Seaweed (chemistry)
• Signal Transduction
• Skin (drug effects, metabolism, pathology, radiation effects)
• Skin Aging (drug effects)
• Ultraviolet Rays