The enzymatic poly(gallic acid) reduces pro-inflammatory cytokines in vitro, a potential application in inflammatory diseases.

Abstract

Cytokines like IL-6, TNF-α, and IL-1β are important mediators of inflammation in many inflammatory diseases, as well as in cellular processes like cell proliferation and cell adhesion. Finding new molecules that decrease cell proliferation, adhesion (inflammatory infiltrate), and pro-inflammatory cytokine release could help in the treatment of many inflammatory diseases. The naturally derived poly(gallic acid) (PGAL), produced enzymatically from gallic acid in aqueous medium, is a non-toxic, thermostable multiradical polyanion that is antioxidant and has potential biomedical uses. Experimental evidence has demonstrated that PGAL reduces pro-inflammatory cytokines, which are the target of some inflammatory diseases. PGAL decreased IL-6, TNF-α, and IL-1β production in human monocytes exposed to PMA without affecting cell viability. Additionally, PGAL reduced cell proliferation by affecting the transition from the S phase to the G2 phase of the cell cycle. Cell adhesion experiments showed that PMA-induced cell adhesion was diminished with the presence of PGAL, particularly at a concentration of 200 μg/mL. These properties of PGAL show a potential use for treating inflammatory diseases, such as psoriasis or arthritis.

• Authors: Yessica Zamudio-Cuevas, Marco A Andonegui-Elguera, Ariadna Aparicio-Juárez, Edson Aguillón-Solís, Karina Martínez-Flores, Erika Ruvalcaba-Paredes, Cristina Velasquillo-Martínez, Clemente Ibarra, Valentín Martínez-López, Marwin Gutiérrez, Roeb García-Arrazola, Carmen G Hernández-Valencia, Alejandra Romero-Montero, Miguel A Hernández-Valdepeña, Miquel Gimeno, Roberto Sánchez-Sánchez
• Journal: Inflammation (Inflammation) Vol. 44 Issue 1 Pg. 174-185 (Feb 2021) ISSN: 1573-2576 [Electronic] United States
• PMID: 32803665 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Inflammation Mediators
• Polylysine
• Polyglutamic Acid
• poly(glutamic acid-lysine)

Topics

• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Cytokines (antagonists & inhibitors, metabolism)
• Dose-Response Relationship, Drug
• HCT116 Cells
• HT29 Cells
• Humans
• Inflammation (drug therapy, metabolism)
• Inflammation Mediators (antagonists & inhibitors, metabolism)
• Polyglutamic Acid (analogs & derivatives, pharmacology, therapeutic use)
• Polylysine (analogs & derivatives, pharmacology, therapeutic use)
• THP-1 Cells