Abstract | BACKGROUND: METHODS: CiRS-7 expression was confirmed in 40 paired OC and normal adjacent tissues from 40 OC patients with different TNM stages, lymph node metastasis status and overall survival rate, also 5 different OC cell lines by qRT-PCR. Effects of ciRS-7 silence on OC cell phenotypes were determined in OC cells and Xenograft mouse model. StarBase was used to predict binding sites between ciRS-7 and micRNAs. Pearson correlation analysis and RNA-immunoprecipitation assay were used to determine the association between genes. Point mutation and rescue experiments were applied for molecular mechanism investigation. RESULTS: CiRS-7 expression was significantly higher in OC cells and tissues, which was significantly associated with the TNM stages, lymph node metastasis status and overall survival rate in OC patients. CiRS-7 silence inhibited OC cell growth and metastasis. CiRS-7 sponged miR-641 to up-regulate ZEB1 and MDM2 expression in OC development. CONCLUSION: CiRS-7 serves as a competing endogenous RNA of miR-641 that promoted cell growth and metastasis in OC, via regulating ZEB1 and MDM2-mediated EMT. High ciRS-7 expression was a poor prognosis of TNM stages, lymph node metastasis status and overall survival rate in OC patients. Targeting ciRS-7/miR-641/ZEB1 or ciRS-7/miR-641/MDM2 axis may be a novel diagnostic, prognostic and therapeutic strategy for OC.
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Authors | Fenghua Zhang, Yun Xu, Wenfeng Ye, Jingting Jiang, Changping Wu |
Journal | Bioscience reports
(Biosci Rep)
Vol. 40
Issue 7
(07 31 2020)
ISSN: 1573-4935 [Electronic] England |
PMID | 32667627
(Publication Type: Journal Article)
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Copyright | © 2020 The Author(s). |
Chemical References |
- Biomarkers, Tumor
- MIRN641 microRNA, human
- MicroRNAs
- RNA, Long Noncoding
- ZEB1 protein, human
- Zinc Finger E-box-Binding Homeobox 1
- long non-coding RNA CDR1AS, human
- MDM2 protein, human
- Proto-Oncogene Proteins c-mdm2
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Topics |
- Animals
- Biomarkers, Tumor
(metabolism)
- Carcinogenesis
(genetics)
- Cell Line, Tumor
- Epithelial-Mesenchymal Transition
(genetics)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Lymphatic Metastasis
(genetics)
- Male
- Mice
- MicroRNAs
(metabolism)
- Neoplasm Staging
- Ovarian Neoplasms
(diagnosis, genetics, mortality, surgery)
- Ovariectomy
- Ovary
(pathology, surgery)
- Prognosis
- Proto-Oncogene Proteins c-mdm2
(genetics)
- RNA, Long Noncoding
(metabolism)
- Survival Rate
- Up-Regulation
- Xenograft Model Antitumor Assays
- Zinc Finger E-box-Binding Homeobox 1
(genetics)
|