Ethanol extract of Pycnoporus sanguineus relieves the dextran sulfate sodium-induced experimental colitis by suppressing helper T cell-mediated inflammation via apoptosis induction.

Abstract

Inflammatory bowel disease (IBD) is a chronic relapsing inflammation involving the gut system, and disequilibrium of T helper (Th) cell paradigm has been recognized as critical pathogenesis. Pycnoporus sanguineus (L.) Murrill is a species of the white-rot basidiomycetes listed as food- and cosmetic-grade microorganisms. In this study, anti-inflammatory activity of the ethanol extract from P. sanguineus (PSE) was investigated in dextran sulfate sodium (DSS)-induced experimental colitis model. PSE recovered the DSS-caused weight loss, reversed the colon shortening, and ameliorated the histopathological lesion in colon, resulting in lower disease activity index (DAI). Levels of serumal lipopolysaccharide (LPS), colonic myeloperoxidase (MPO) in the colitis-suffering mice were declined by PSE treatment. PSE also improved the mucosal integrity by enhancing the expression of tight junction and adherens junction proteins in the colon, including ZO-1, occludin, claudin-1, and E-cadherin. Besides, PSE reduced helper T cells (Th) in the colon, together with an evident decrease of several Th cell-related cytokines. Moreover, it was found that in vitro, PSE suppressed T cells and the Th subset upon Concanavalin A (ConA)-stimulation by inducing apoptosis. In summary, PSE displayed a remission on the colitis-related inflammation, which would possibly rely on the epithelial barrier restoration by suppressing Th cells via apoptosis induction, highlighting a promising potential in the treatment for IBD.

• Authors: Xiaohong Chen, Muxia Li, Dan Li, Ting Luo, Yizhen Xie, Liang Gao, Yifan Zhang, Shaodan Chen, Shunxian Li, Guoxin Huang, Wenzhi Li, Jiyan Su, Xiaoping Lai
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 127 Pg. 110212 (Jul 2020) ISSN: 1950-6007 [Electronic] France
• PMID: 32422567 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Plant Extracts
• Ethanol
• Dextran Sulfate

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Colitis (drug therapy, pathology)
• Cytokines (metabolism)
• Dextran Sulfate (toxicity)
• Disease Models, Animal
• Ethanol (chemistry)
• Inflammation (drug therapy, pathology)
• Inflammatory Bowel Diseases (drug therapy, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Plant Extracts (pharmacology)
• Polyporaceae (chemistry)
• T-Lymphocytes, Helper-Inducer (immunology)