Morin inhibits Listeria monocytogenes virulence in vivo and in vitro by targeting listeriolysin O and inflammation.

Abstract

BACKGROUND:

Listeria monocytogenes (L. monocytogenes) is a global opportunistic intracellular pathogen that can cause many infections, including meningitis and abortion in humans and animals; thus, L. monocytogenes poses a great threat to public safety and the development of the aquaculture industry. The isolation rate of Listeria monocytogenes in fishery products has always been high. And the pore-forming toxin listeriolysin O (LLO) is one of the most important virulence factors of L. monocytogenes. LLO can promote cytosolic bacterial proliferation and help the pathogen evade attacks from the host immune system. In addition, L. monocytogenes infection can trigger a series of severe inflammatory reactions.

RESULTS:

Here, we further confirmed that morin lacking anti-Listeria activity could inhibit LLO oligomerization. We also found that morin can effectively alleviate the inflammation induced by Listeria in vivo and in vitro and exerted an obvious protective effect on infected cells and mice.

CONCLUSIONS:

Morin does not possess anti-Listeria activity, neither does it interfere with secretion of LLO. However, morin inhibits oligomerisation of LLO and morin does reduce the inflammation caused during Listeria infection.

• Authors: Gen Li, Guizhen Wang, Meng Li, Li Li, Hongtao Liu, Meiyang Sun, Zhongmei Wen
• Journal: BMC microbiology (BMC Microbiol) Vol. 20 Issue 1 Pg. 112 (05 12 2020) ISSN: 1471-2180 [Electronic] England
• PMID: 32398085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Toxins
• Flavonoids
• Heat-Shock Proteins
• Hemolysin Proteins
• morin
• hlyA protein, Listeria monocytogenes

Topics

• Animals
• Bacterial Toxins (chemistry)
• Cell Line
• Disease Models, Animal
• Flavonoids (administration & dosage, pharmacology)
• Gene Expression Regulation, Bacterial (drug effects)
• Heat-Shock Proteins (chemistry, drug effects)
• Hemolysin Proteins (chemistry, drug effects)
• Humans
• Listeria monocytogenes (drug effects, enzymology, growth & development, pathogenicity)
• Listeriosis (drug therapy)
• Mice
• Protein Multimerization (drug effects)
• Virulence (drug effects)