Abstract | AIMS: METHODS: Screening test pilot study recruited 90 participants from a tertiary hospital in Lima, Peru. Participants were grouped by diabetes and neuropathy status. Tears collected on Schirmer strips, and proteins measured by both ELISA and multiplex-bead assay. Corneal sensitivity was measured by aesthesiometry, and DPN by vibration perception threshold testing. RESULTS: There were 89 participants included in the analysis. The mean age was 55.7 ± 1.46, and 58.4% were female. MMP-9 and TGF-alpha concentrations were higher in participants with DPN versus diabetes alone, though not significant. Aesthesiometry was decreased in individuals with DPN when compared to participants with diabetes alone (p < 0.01) and normal controls (p < 0.01). Optimal cutoff point for aesthesiometry was found to be 5.8 cm, with 79% sensitivity and 75% specificity. CONCLUSIONS: Tears are an insufficient standalone tool for detecting DPN based on the biomarkers analyzed. Aesthesiometry is a simple, inexpensive, and accurate method to assess corneal damage associated with moderate-severe DPN, and its integration into screening practices has potential to improve detection of DPN in poor-resource settings.
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Authors | Meera F Iyengar, Luis F Soto, David Requena, Andrea O Ruiz-Alejos, Yvonne Huaylinos, Ricardo Velasquez, Antonio Bernabe-Ortiz, Robert H Gilman |
Journal | Diabetes research and clinical practice
(Diabetes Res Clin Pract)
Vol. 163
Pg. 108143
(May 2020)
ISSN: 1872-8227 [Electronic] Ireland |
PMID | 32283127
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Biomarkers
- Cornea
(pathology)
- Diabetes Mellitus, Type 2
(complications)
- Diabetic Neuropathies
(diagnosis, pathology)
- Female
- Humans
- Male
- Middle Aged
- Pilot Projects
- Tears
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