lncRNA TUG1 regulates ulcerative colitis through miR-142-5p/SOCS1 axis.

Abstract

Ulcerative colitis (UC) is a long-lasting inflammation disease which finally results in ulcer of the colon and rectum. The long non-coding RNA (lncRNA) TUG1 has been described to target miR-142 and regulate its expression. In current study, we evaluated the effects of long non-coding RNA TUG1 on cell injury and inflammatory cytokine production using a TNFα-treated HT-29 cells model. We monitored the level of TUG1 in colonic mucosa tissue of UC patients and in TNF-α-treated HT-29 cells. We investigated the effects of TUG1 on miR-142-5p and SOCS1expression, cell viability, lactate dehydrogenase (LDH) release, production of nitrite and PGE2 after TNF-α treatment in HT-29 cells. We also investigated the effects of TUG1 on TNF-α-induced IL-6, IL-8 and IL-1β expression in HT-29 cells. We detected down-regulated TUG1 level in colonic mucosa tissue of UC patients and in TNF-α-treated HT-29 cells. Overexpression of TUG1 enhanced cell viability, decreased LDH release, decreased nitrite and PGE2 production after TNF-α treatment in HT-29 cells. TUG1 prevented IL-1β, IL-6 and IL-8 production in TNF-α-treated cells. TUG1 targeted miR-142-5p and inhibited its expression while enhanced SOCS1 expression. Overexpression of miR-142-5p abolished TUG1-mediated inhibition of TNF-induced inflammatory cytokines production. TUG1 negatively regulated inflammation in ulcerative colitis through miR-142-5p/SOCS1 axis.

• Authors: Jing Han, Yawei Li, Baolian Zhang, Hong Liu, Mengyao Wu, Xiaolan Zhang
• Journal: Microbial pathogenesis (Microb Pathog) Vol. 143 Pg. 104139 (Jun 2020) ISSN: 1096-1208 [Electronic] England
• PMID: 32173492 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Interleukins
• MIRN142 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• SOCS1 protein, human
• Suppressor of Cytokine Signaling 1 Protein
• TUG1 long noncoding RNA, human

Topics

• Blotting, Western
• Colitis, Ulcerative (metabolism)
• Enzyme-Linked Immunosorbent Assay
• HT29 Cells
• Humans
• Inflammation (metabolism)
• Interleukins (metabolism)
• Intestinal Mucosa (metabolism)
• MicroRNAs (metabolism)
• RNA, Long Noncoding (metabolism)
• Real-Time Polymerase Chain Reaction
• Suppressor of Cytokine Signaling 1 Protein (metabolism)