Parkinson's disease is caused due to
free radical generation in dopamine neurons leading to oxidative stress induced damage. The aim of this work was to ameliorate the
free radical induced oxidative stress in rats by using
TPGS (Tocopheryl
polyethylene glycol 1000 succinate) loaded
rutin nanoemulsion after
oral administration. For this purpose, pharmacokinetic and pharmacodynamics studies were performed in albino wistar rats. Various behavioural tests (photoactometer test, rota rod, akinesia and
catalepsy) and biochemical estimations for determination of GSH,
TBARS and SOD were carried out. The results showed an increase in relative bioavailability of
rutin after
oral administration of nanoemulsion as compared to pure drug
suspension. The AUC and Cmax of
rutin nanoemulsion after
oral administration were 1.8-fold and 1.9-fold higher than those of drug
suspension respectively. Pharmacodynamic studies have shown good results with the
rutin nanoemulsion than pure drug
suspension. The rats treated with the
rutin nanoemulsion exhibited significantly greater locomotor activity, better muscle coordination and improvement in cataleptic behaviour than the normal and
haloperidol-induced rats (p < 0.001).Treatment with
rutin suspension and
rutin nanoemulsion helped in improving the stressed condition by increasing the levels of GSH, SOD and decrease in MDA levels in the brain. Anticancer activity was observed in a dose-dependent manner from 1 to 100 μg/ml. IC50 values for
rutin suspension and
rutin NE were found to be 36.7 and 25.4 μg/ml respectively. The
rutin nanoemulsion has proven to be beneficial in ameliorating oxidative stress.