Abstract |
Autoimmune thyroiditis (AIT)-related brain damage is one of most severe extrathyroidal manifestations of AIT, but the mechanism remains unclear. In this study, we confirmed that protein disulfide-isomerase A3 (PDIA3) is expressed in both thyroid and brain tissues of mouse, and found the significantly increased serum levels of anti-PDIA3 antibody (PDIA3Ab) in classical mouse models of thyroiditis. In addition, we investigated the PDIA3-specific autoimmune reaction in thyroid and brain tissues in a mouse model with high-serum PDIA3Ab induced by immunization with recombinant PDIA3 protein. PDIA3-immunized mice had elevated serum thyrotropin and impaired learning and memory. PDIA3-expressing cells had IgG deposition, and IgG colocalized with C3 in the thyroid and brain tissues of PDIA3-immunized mice, resulting in membrane attack complex formation. Our results suggest that PDIA3 protein may be a common autoantigen shared by the thyroid and brain tissues and involve in the thyroidal and intracerebral damage through activating complement system.
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Authors | Wenqing Yang, Yang Xiang, Hongmei Zhang, Zhongyan Shan, Jing Li, Weiping Teng |
Journal | Clinical immunology (Orlando, Fla.)
(Clin Immunol)
Vol. 212
Pg. 108350
(03 2020)
ISSN: 1521-7035 [Electronic] United States |
PMID | 31982645
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020. Published by Elsevier Inc. |
Chemical References |
- Autoantibodies
- Autoantigens
- Pdia3 protein, mouse
- Protein Disulfide-Isomerases
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Topics |
- Animals
- Apoptosis
(immunology)
- Autoantibodies
(immunology)
- Autoantigens
(immunology)
- Brain
(immunology, pathology, physiopathology, ultrastructure)
- Disease Models, Animal
- Encephalitis
(immunology, pathology, physiopathology)
- Excitatory Postsynaptic Potentials
(physiology)
- Hashimoto Disease
(immunology, pathology, physiopathology)
- Long-Term Potentiation
(physiology)
- Maze Learning
- Mice
- Protein Disulfide-Isomerases
(immunology)
- Thyroid Gland
(immunology, pathology)
- Thyroiditis, Autoimmune
(immunology, pathology, physiopathology)
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