Microbial burden and inflammasome activation in amniotic fluid of patients with preterm prelabor rupture of membranes.

Abstract

Background Intra-amniotic inflammation, which is associated with adverse pregnancy outcomes, can occur in the presence or absence of detectable microorganisms, and involves activation of the inflammasome. Intra-amniotic inflammasome activation has been reported in clinical chorioamnionitis at term and preterm labor with intact membranes, but it has not yet been investigated in women with preterm prelabor rupture of membranes (preterm PROM) in the presence/absence of detectable microorganisms. The aim of this study was to determine whether, among women with preterm PROM, there is an association between detectable microorganisms in amniotic fluid and intra-amniotic inflammation, and whether intra-amniotic inflammasome activation correlates with microbial burden. Methods Amniotic fluids from 59 cases of preterm PROM were examined for the presence/absence of microorganisms through culture and 16S ribosomal RNA (rRNA) gene quantitative real-time polymerase chain reaction (qPCR), and concentrations of interleukin-6 (IL-6) and ASC [apoptosis-associated spec-like protein containing a caspase recruitment domain (CARD)], an indicator of inflammasome activation, were determined. Results qPCR identified more microbe-positive amniotic fluids than culture. Greater than 50% of patients with a negative culture and high IL-6 concentration in amniotic fluid yielded a positive qPCR signal. ASC concentrations were greatest in patients with high qPCR signals and elevated IL-6 concentrations in amniotic fluid (i.e. intra-amniotic infection). ASC concentrations tended to increase in patients without detectable microorganisms but yet with elevated IL-6 concentrations (i.e. sterile intra-amniotic inflammation) compared to those without intra-amniotic inflammation. Conclusion qPCR is a valuable complement to microbiological culture for the detection of microorganisms in the amniotic cavity in women with preterm PROM, and microbial burden is associated with the severity of intra-amniotic inflammatory response, including inflammasome activation.

• Authors: Kevin R Theis, Roberto Romero, Kenichiro Motomura, Jose Galaz, Andrew D Winters, Percy Pacora, Derek Miller, Rebecca Slutsky, Violetta Florova, Dustyn Levenson, Robert Para, Aneesha Varrey, Marian Kacerovsky, Chaur-Dong Hsu, Nardhy Gomez-Lopez
• Journal: Journal of perinatal medicine (J Perinat Med) Vol. 48 Issue 2 Pg. 115-131 (Feb 25 2020) ISSN: 1619-3997 [Electronic] Germany
• PMID: 31927525 (Publication Type: Journal Article)

Chemical References

• CARD Signaling Adaptor Proteins
• Inflammasomes
• PYCARD protein, human
• RNA, Ribosomal, 16S

Topics

• Adult
• Amniotic Fluid (metabolism, microbiology)
• CARD Signaling Adaptor Proteins (metabolism)
• Cross-Sectional Studies
• Female
• Fetal Membranes, Premature Rupture (metabolism, microbiology)
• Humans
• Inflammasomes (metabolism)
• Pregnancy
• RNA, Ribosomal, 16S (analysis)
• Real-Time Polymerase Chain Reaction
• Retrospective Studies
• Young Adult