[LIGHT/ TNFSF14 alleviates cisplatin-induced acute kidney injury in mice and its mechanism].

Abstract

Objective To investigate the role of LIGHT/TNFSF14 (TNF superfamily protein 14) in cisplatin-induced acute kidney injury (Cis-AKI) in mice and explore the underlying mechanism. Methods Male wild-type (WT) and LIGHT gene knockout (LIGHT-/-) C57BL/6 mice were selected and divided into four groups: saline- and cisplatin-treated WT mice, saline- and cisplatin-treated LIGHT-/- mice. The cisplatin groups were given a single intraperitoneal injection of cisplatin (20 mg/kg, 200 μL), and the saline groups were injected with equal volume of normal saline (9 g/L). After 72 hours, the mice were sacrificed, blood was taken from the eyeball, and kidney tissues were collected. Blood urea nitrogen (BUN) and serum creatinine (Scr) were measured by automatic biochemical analyzer. HE staining was used to detect the histopathological changes of kidney tissues, The mRNA levels of LIGHT, kidney injury molecule 1 (KIM-1), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), and tumor necrosis factor (TNF-α) were detected by real-time quantitative PCR. The protein levels of LIGHT, Bcl2, BAX and cytochrome C were detected by Western blot analysis or immunohistochemical staining. Results Compared with saline-treated WT mice, the expression of LIGHT in renal tissue of cisplatin-treated WT mice significantly increased. Compared with cisplatin-treated WT mice, the kidney injury in cisplatin-treated LIGHT-/- mice was more serious; BUN and Scr increased; and the pathological damage of kidney tissue was more obvious. Moreover, the mRNA levels of IL-6, MCP-1 and TNF-α, as well as the protein levels of BAX and cytochrome C increased, while the protein levels of Bcl2 decreased. Conclusion LIGHT plays a protective role in Cis-AKI, which may be related to down-regulated secretion of inflammatory factors and decreased apoptosis.

• Authors: Yan Yang, Yu Zhong, Li Meng, Pan Xie, Guilian Xu, Kanfu Peng
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 35 Issue 10 Pg. 897-902 (Oct 2019) ISSN: 1007-8738 [Print] China
• PMID: 31814566 (Publication Type: Journal Article)

Chemical References

• Tnfsf14 protein, mouse
• Tumor Necrosis Factor Ligand Superfamily Member 14
• Creatinine
• Cisplatin

Topics

• Acute Kidney Injury (chemically induced)
• Animals
• Apoptosis
• Blood Urea Nitrogen
• Cisplatin (toxicity)
• Creatinine (blood)
• Kidney (pathology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Tumor Necrosis Factor Ligand Superfamily Member 14 (metabolism)