The protective effect of Sargassum horneri against particulate matter-induced inflammation in lung tissues of an in vivo mouse asthma model.

Abstract

Sargassum horneri is an edible brown seaweed with potential anti-inflammatory properties. The present study was designed to evaluate the anti-inflammatory properties of S. horneri using an in vivo mouse asthma model following exposure to particulate matter (PM). 7-8 week old BALB/c mice (20-25 g) were randomly divided into seven groups (n = 4) as follows: 1: no treatment, 2: OVA (ovalbumin) + PM, 3: OVA + PM + SHE (S. horneri ethanol extract) 200 mg kg-1, 4: OVA + PM + SHE 400 mg kg-1, 5: OVA + PM + prednisone 5 mg kg-1, 6: OVA only, and 7: PM only. All mice (except healthy controls) were sensitized on the first day by intraperitoneal injection of 10 μg OVA and 2 mg Al(OH)3 in 200 μL of saline. Starting from day 15, mice (except groups 1 and 6) were exposed to sonicated PM (5 mg m-3, 30 min day-1) through a nebulizer daily for 7 consecutive days. Mice exposed to PM and OVA showed up-regulated expression of MAPKs and pro-inflammatory cytokine production in the lungs. Furthermore, PM-exposed lungs had significantly reduced expression of Nrf2 and HO-1 genes. However, oral administration of the SHE reduced the phosphorylation levels of MAPKs, iNOS and COX2 expression levels, and mRNA expression levels of pro-inflammatory cytokines. In addition, SHE treated group mice had up-regulated anti-oxidant gene expression levels in the lungs compared to group 2. These findings demonstrate that oral administration of the SHE re-establishes PM-induced inflammation and oxidative stress in the lungs. Taken together, the SHE has therapeutic potential in preventing PM-induced inflammation and oxidative stress.

• Authors: K K Asanka Sanjeewa , Thilin U Jayawardena , Hyo Geun Lee , Kalahe Hewage Iresha Nadeeka Madushani Herath , Youngheun Jee , You-Jin Jeon
• Journal: Food & function (Food Funct) Vol. 10 Issue 12 Pg. 7995-8004 (Dec 11 2019) ISSN: 2042-650X [Electronic] England
• PMID: 31793623 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Membrane Proteins
• NF-E2-Related Factor 2
• Nfe2l2 protein, mouse
• Particulate Matter
• Plant Extracts
• Protective Agents
• Nitric Oxide Synthase Type II
• Heme Oxygenase-1
• Hmox1 protein, mouse

Topics

• Animals
• Asthma (etiology, genetics, immunology, prevention & control)
• Cytokines
• Female
• Heme Oxygenase-1 (genetics, immunology)
• Lung (drug effects, immunology)
• Male
• Membrane Proteins (genetics, immunology)
• Mice
• Mice, Inbred BALB C
• NF-E2-Related Factor 2 (genetics, immunology)
• Nitric Oxide Synthase Type II (genetics, immunology)
• Particulate Matter (adverse effects)
• Plant Extracts
• Protective Agents (administration & dosage)
• Sargassum (chemistry)