Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: Present study was undertaken to investigate the effect of TJ treatment with fresh RS against intrahepatic cholestasis in rats and provide an experimental basis for the underlying mechanism of TJ therapy. MATERIALS AND METHODS: RESULTS: After TJ treatment with fresh RS, the abnormally elevated levels of serum AST, ALT, ALP, DBIL, TBIL and TBA, as well as hepatic MDA and NO at 108 h were reduced significantly (versus model group, P < 0.01). The hepatic body ratio, bile flow and hepatic pathological change of cholestatic rats at 108 h in TJ group were restored when compared with those of model group. Thirty-one compounds including lactones, flavonoids and phenolic acids were identified and determined by GC-MS analysis. The content of protoanemonin in fresh RS (9.49%) was about 25-fold higher than that in dried RS (0.38%). CONCLUSIONS: TJ treatment with fresh RS exhibited good therapeutic effect on ANIT-induced intrahepatic cholestasis in rats, which may be due to the attenuated oxidative stress in the liver tissue. It is rational for the ancients to choose fresh RS as TJ herbal patches because of its abundant protoanemonin with the character of irritant. The qualitative and quantitative results of GC-MS analysis provided the chemical basis of TJ therapy with fresh RS, which can be regarded as a simple and efficient method for the treatment of cholestasis hepatitis.
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Authors | Zhiyong Zhang, Yiru Miao, Min Xu, Wenming Cheng, Chuanyan Yang, Xiangjian She, Qianqian Geng, Qunlin Zhang |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 248
Pg. 112310
(Feb 10 2020)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 31629027
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers
- 1-Naphthylisothiocyanate
- 2-naphthylisothiocyanate
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Topics |
- 1-Naphthylisothiocyanate
- Animals
- Biomarkers
(blood)
- Cholestasis, Intrahepatic
(blood, chemically induced, pathology, therapy)
- Disease Models, Animal
- Lipid Peroxidation
- Liver
(metabolism, pathology)
- Male
- Moxibustion
- Oxidative Stress
- Ranunculus
- Rats, Sprague-Dawley
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