Ibuprofen attenuates interleukin-1β-induced inflammation and actin reorganization via modulation of RhoA signaling in rabbit chondrocytes.

Abstract

Ibuprofen, a medication in the nonsteroidal anti-inflammatory drug class, is widely used for treating inflammatory diseases such as osteoarthritis. It has been shown in recent years that ibuprofen has a strong effect on Ras homolog gene family, member A (RhoA) inhibition in multiple cell types. Our previous finding also demonstrated that interleukin-1β (IL-1β) increases filamentous actin (F-actin) of chondrocytes via RhoA pathway. Therefore, we hypothesized that ibuprofen may suppress the IL-1β-induced F-actin upregulation in chondrocytes by inhibiting RhoA pathway. To this end, in this study, articular chondrocytes from New Zealand White rabbits were pretreated with 500 μM ibuprofen for 2 h, then with 10 ng/ml IL-1β for 24 h. Results showed that pretreatment with ibuprofen inhibited the IL-1β-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production, protected the chondrocyte phenotype from IL-1β stimulation, and inhibited the IL-1β-induced actin remodeling via RhoA signaling modulation. In conclusion, ibuprofen showed not only anti-inflammatory function, but also RhoA inhibition in articular chondrocytes.

• Authors: Rui Li, Xiongbo Song, Gaoming Li, Zhen Hu, Li Sun, Cheng Chen, Liu Yang
• Journal: Acta biochimica et biophysica Sinica (Acta Biochim Biophys Sin (Shanghai)) Vol. 51 Issue 10 Pg. 1026-1033 (Sep 06 2019) ISSN: 1745-7270 [Electronic] China
• PMID: 31553428 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2019. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Actins
• Anti-Inflammatory Agents, Non-Steroidal
• Interleukin-1beta
• rhoA GTP-Binding Protein
• Ibuprofen

Topics

• Actins (immunology)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Cells, Cultured
• Chondrocytes (drug effects, immunology, pathology)
• Ibuprofen (pharmacology)
• Inflammation (drug therapy, immunology, pathology)
• Interleukin-1beta (immunology)
• Rabbits
• rhoA GTP-Binding Protein (immunology)