Effects of traditional Chinese medicine formula Le-Cao-Shi on hepatitis B: In vivo and in vitro studies.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Formula Le-Cao-Shi (LCS) is a traditional Chinese medicine (TCM), which has long been used as a folk remedy against hepatitis B in China. The present study was conducted to evaluate the anti-hepatitis B effects of aqueous extract of LCS in vivo and in vitro.

MATERIALS AND METHOD:

we investigated the anti-HBV effects of LCS in vivo and in vitro with duck hepatitis B model and HepG2.2.15 cell line model, respectively. The serologic and cellular biomarkers and the histopathological changes were examined.

RESULTS:

By a duck hepatitis B model, the extract of LCS was found to restrain the expressions of duck hepatitis B surface antigen (DHBsAg), hepatitis B e antigen (DHBeAg), and HBV-DNA (DHBV-DNA). Moreover, LCS could decrease the levels of aspartate and alanine aminotransferases (AST and ALT) and ameliorate duck liver histological lesions. Correspondingly, in a HepG2.2.15 cellular model, LCS could also significantly inhibit the secretions of HBsAg and HBeAg.

CONCLUSION:

LCS exerted potent anti-hepatitis effects against the infection of HBV. The above results demonstrated the first-hand experimental evidences for the anti-hepatitis B efficiency of LCS. Our study provides a basis for further exploration and development of this promising compound prescription to treat hepatitis B disease.

• Authors: Qing Zhao, Xia Ren, Min Chen, Shi-Jun Yue, Meng-Qi Zhang, Kai-Xian Chen, Yue-Wei Guo, Chang-Lun Shao, Chang-Yun Wang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 244 Pg. 112132 (Nov 15 2019) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 31381954 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• DNA, Viral
• Drugs, Chinese Herbal
• Hepatitis B Surface Antigens
• Hepatitis B e Antigens
• Le-Cao-Shi

Topics

• Animals
• Antiviral Agents (pharmacology, therapeutic use)
• Cell Line, Tumor
• Cell Survival (drug effects)
• DNA, Viral
• Drugs, Chinese Herbal (therapeutic use)
• Ducks
• Hepatitis B (drug therapy, immunology, pathology, virology)
• Hepatitis B Surface Antigens (immunology)
• Hepatitis B Virus, Duck (drug effects, genetics, immunology)
• Hepatitis B e Antigens (immunology)
• Hepatitis, Viral, Animal (drug therapy, immunology, pathology, virology)
• Humans
• Liver (drug effects, pathology)
• Medicine, Chinese Traditional