Effect of bisphenol-A (BPA) on placental biomarkers for inflammation, neurodevelopment and oxidative stress.

Abstract

Background Bisphenol-A (BPA) is a widespread pollutant whose effects on pregnant women are poorly understood. Therefore, we investigated the effects of BPA on basal and bacteria-stimulated production of proinflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor-α (TNF-α) and IL-6], anti-inflammatory mediators [soluble glycoprotein 130 (sgp) 130, heme oxidase-1 (HO-1) and IL-10] and biomarkers for neurodevelopment [brain-derived neurotrophic factor (BDNF)], and oxidative stress [8-isoprostane (8-IsoP)] by the placenta. Methods Placental explant cultures were treated with BPA (0-10,000 nM) in the presence or absence of 107 colony-forming unit (CFU)/mL heat-killed Escherichia coli for 24 h. Biomarker concentrations in conditioned medium were quantified by the enzyme-linked immunosorbent assay (ELISA). Results Under basal conditions, IL-1β and IL-6 production was enhanced by BPA in a dose-dependent manner. Sgp130, a soluble receptor that reduces IL-6 bioactivity, was suppressed by BPA at 1000-10,000 nM. BPA also enhanced BDNF production at 1000 and 10,000 nM, and 8-IsoP expression at 10 and 100 nM. For bacteria-treated cultures, BPA increased IL-6 production at 100 nM and reduced sgp130 at 1000 nM but had no effect on IL-1β, TNF-α, BDNF, HO-1, 8-IsoP or IL-10 production. Conclusion BPA may increase placental inflammation by promoting IL-1β and IL-6 but inhibiting sgp130. It may also disrupt oxidative balance and neurodevelopment by increasing 8-IsoP and BDNF production.

• Authors: Yuko Arita, Hyeon Jeong Park, Aisling Cantillon, Darios Getahun, Ramkumar Menon, Morgan R Peltier
• Journal: Journal of perinatal medicine (J Perinat Med) Vol. 47 Issue 7 Pg. 741-749 (Sep 25 2019) ISSN: 1619-3997 [Electronic] Germany
• PMID: 31339859 (Publication Type: Journal Article)

Chemical References

• Air Pollutants, Occupational
• Benzhydryl Compounds
• Biomarkers
• Brain-Derived Neurotrophic Factor
• Cytokines
• Estrogens, Non-Steroidal
• Phenols
• bisphenol A

Topics

• Air Pollutants, Occupational (adverse effects, metabolism)
• Benzhydryl Compounds (adverse effects, metabolism)
• Biomarkers (metabolism)
• Brain-Derived Neurotrophic Factor (metabolism)
• Colony Count, Microbial (methods)
• Cytokines (classification, metabolism)
• Escherichia coli (growth & development)
• Estrogens, Non-Steroidal (adverse effects, metabolism)
• Female
• Humans
• Inflammation (chemically induced, metabolism)
• Oxidative Stress (drug effects)
• Phenols (adverse effects, metabolism)
• Placenta (drug effects, immunology, metabolism)
• Pregnancy