Abstract |
Most of current influenza virus vaccines fail to develop a strong immunity at lung mucosae (site of viral entry) due to sub-optimal vaccination protocols (e.g. inactivated virus administered by parenteral injections). Mucosal immunity could be improved by using locally-delivered vaccines containing appropriate adjuvants. Here we show, in a mouse model, that inclusion of silver nanoparticles (AgNPs) in virus-inactivated flu vaccine resulted in reduction of viral loads and prevention of excessive lung inflammation following influenza infection. Concomitantly, AgNPs enhanced specific IgA secreting plasma cells and antibodies titers, a hallmark of successful mucosal immunity. Moreover, vaccination in the presence of AgNPs but not with gold nanoparticles, protected mice from lethal flu. Compared with other commercial adjuvants ( squalene/oil-based emulsion) or silver salts, AgNPs stimulated stronger antigen specific IgA production with lower toxicity by promoting bronchus-associated lymphoid tissue (BALT) neogenesis, and acted as a bona fide mucosal adjuvant.
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Authors | Daniel Sanchez-Guzman, Pierre Le Guen, Berengere Villeret, Nuria Sola, Remi Le Borgne, Alice Guyard, Alix Kemmel, Bruno Crestani, Jean-Michel Sallenave, Ignacio Garcia-Verdugo |
Journal | Biomaterials
(Biomaterials)
Vol. 217
Pg. 119308
(10 2019)
ISSN: 1878-5905 [Electronic] Netherlands |
PMID | 31279103
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2019 Elsevier Ltd. All rights reserved. |
Chemical References |
- Adjuvants, Immunologic
- Immunoglobulin A
- Influenza Vaccines
- Silver
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Animals
- Bronchi
(immunology)
- Dogs
- Germinal Center
(drug effects, metabolism)
- Humans
- Immunity, Mucosal
(drug effects)
- Immunoglobulin A
(metabolism)
- Inflammation
(pathology)
- Influenza A Virus, H1N1 Subtype
(drug effects, immunology)
- Influenza Vaccines
(immunology)
- Influenza, Human
(immunology)
- Lymphoid Tissue
(drug effects, immunology)
- Madin Darby Canine Kidney Cells
- Metal Nanoparticles
(chemistry, ultrastructure)
- Mice, Inbred C57BL
- Orthomyxoviridae Infections
(immunology, prevention & control, virology)
- Silver
(chemistry)
- Vaccination
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