Present study evaluates the protective effect of
mollugin against Klebsiella
pneumonia (KP) and also postulates the possible mechanism of its action. Klebsiella pneumoniae (2.4 × 108 CFU/ml) was used for the induction of KP. PMNs and WBC count was determined in the blood and bronchoalveolar lavage fluid (BALF) of Klebsiella
pneumonia rat. Level of inflammatory
cytokines in the blood of Klebsiella
pneumonia rat was determined by ELISA methods. Moreover effect of
mollugin was estimated by Western blot assay and RT-PCR method. Result of the study suggests that water content in lung was reduced in the
mollugin treated group compared to
pneumonia control group of rats. Count of PMNs and WBC were found to be reduced in
mollugin treated group compared to
pneumonia control group of rats. Level of inflammatory
cytokines was also found to be reduced in the blood of
mollugin treated group than
pneumonia control group. Moreover treatment with
mollugin attenuates the altered expression of p-MAPK, p-JNK and p-ERK
protein and
mRNA expression of NF-κB in the lung tissues of Klebsiella
pneumonia rat. In conclusion, data of the study reveals that treatment with
mollugin ameliorates Klebsiella
pneumonia rat by reducing the
lung inflammation.
Inflammation of lung tissue was reduced by regulating the NF-κB/MAPK signaling pathway in
mollugin treated group.