Rhamnogalacturonan-I-Type Polysaccharide Purified from Broccoli Exerts Anti-Metastatic Activities Via Innate Immune Cell Activation.

Abstract

To examine the anti-metastatic activities of polysaccharides in broccoli, purified polysaccharides (BCE-I, -II, and -III) were isolated by fractionation of broccoli enzyme extracts and subsequent ethanol precipitation. BCE-I mainly consisted of galactose and arabinose, whereas BCE-II mainly consisted of galacturonic acid and rhamnose, and BCE-III mainly consisted of rhamnose and galactose. Of the three fractions, stimulation of murine peritoneal macrophages by BCE-I showed the greatest enhancement of tumor necrosis factor-α, interleukin (IL)-12, and IL-6 secretion. In addition, intravenous (i.v.) administration of BCE-I enhanced the lethal activity of natural killer (NK) cells on YAC-1 tumor cells significantly and dose-dependently in an ex vivo experiment of NK cell activity. In an experimental model using lung metastasis of Colon26-M3.1 carcinoma cells, prophylactic i.v. and oral administration of BCE-I significantly and dose-dependently inhibited lung metastatic activity. Furthermore, the inhibitory activity of BCE-1 on lung metastasis partially disappeared when NK cell function was removed through treatment of rabbit anti-asialo GM1. These results indicated that BCE-I has potent antitumor metastatic activity, and that its anti-metastatic activity has relevance to the stimulation of NK and other immune cells.

• Authors: Bong-Shin Kwak, Dahyun Hwang, Sue Jung Lee, Hyuk-Jun Choi, Ho-Young Park, Kwang-Soon Shin
• Journal: Journal of medicinal food (J Med Food) Vol. 22 Issue 5 Pg. 451-459 (May 2019) ISSN: 1557-7600 [Electronic] United States
• PMID: 30897013 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Interleukin-6
• Plant Extracts
• Polysaccharides
• rhamnogalacturonan I
• Interleukin-12
• Pectins

Topics

• Animals
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Brassica (chemistry)
• Colonic Neoplasms (immunology, pathology)
• Female
• Humans
• Immunity, Innate (drug effects)
• Interleukin-12 (genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Killer Cells, Natural (drug effects, immunology)
• Lung Neoplasms (immunology, prevention & control, secondary)
• Macrophage Activation (drug effects)
• Macrophages, Peritoneal (drug effects, immunology)
• Mice
• Mice, Inbred BALB C
• Neoplasm Metastasis (immunology, prevention & control)
• Pectins (chemistry, isolation & purification, pharmacology)
• Plant Extracts (chemistry, isolation & purification, pharmacology)
• Polysaccharides (chemistry, isolation & purification, pharmacology)