Abstract | BACKGROUND: METHODS: In this study, we examined the expression of FGD4 in prostate cancer specimens using immunohistochemistry and studied the function of FGD4 in maintaining cell phenotype, behavior and drug sensitivity using overexpression and siRNA-based silencing approaches. We used Mann-Whitney test for comparative analysis of FGD4 expression. RESULTS: Our results show that the expression of FGD4 is upregulated in cancerous prostates compared to the luminal cells in benign prostatic hyperplasia, although the basal cells showed high staining intensities. We noted a gradual increase in the staining intensity of FGD4 with increasing aggressiveness of the disease. Inhibition of expression of FGD4 using siRNAs showed reduced proliferation and cell cycle arrest in G2/M phase of androgen dependent LNCaP-104S and androgen refractory PC-3 cells. Inhibition of FGD4 also resulted in reduced cell migration and CDC42 activities in PC-3 cells whereas, ectopic expression of FGD4 induced cell migration, altered expression of mesenchymal and epithelial markers and activation of CDC42/PAK signaling pathway. Reduced expression of FGD4 improved sensitivity of LNCaP-104S cells to the anti- androgen drug Casodex and PC-3 cells to the microtubule stabilizing drug docetaxel. CONCLUSIONS:
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Authors | Alexia Bossan, Richard Ottman, Thomas Andl, Md Faqrul Hasan, Nupam Mahajan, Domenico Coppola, Ratna Chakrabarti |
Journal | BMC cancer
(BMC Cancer)
Vol. 18
Issue 1
Pg. 1257
(Dec 17 2018)
ISSN: 1471-2407 [Electronic] England |
PMID | 30558664
(Publication Type: Journal Article)
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Chemical References |
- Anilides
- FGD4 protein, human
- Microfilament Proteins
- Nitriles
- RNA, Small Interfering
- Tosyl Compounds
- bicalutamide
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Topics |
- Anilides
(pharmacology)
- Cell Cycle Checkpoints
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Drug Resistance, Neoplasm
- Gene Expression Regulation, Neoplastic
- Humans
- Male
- Microfilament Proteins
(genetics, metabolism)
- Neoplasm Grading
- Nitriles
(pharmacology)
- Phenotype
- Prostatic Neoplasms
(genetics, metabolism, pathology)
- RNA, Small Interfering
(pharmacology)
- Tosyl Compounds
(pharmacology)
- Up-Regulation
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