Diffusion Kurtosis Imaging Reflects Glial Fibrillary Acidic Protein (GFAP), Topo IIα, and O⁶-Methylguanine-DNA Methyltransferase (MGMT) Expression in Astrocytomas.

Abstract

BACKGROUND Astrocytomas are the most common primary brain neoplasms. Biological indicators of astrocytomas can reflect its biological characteristics. The aim of this study was to assess the expression of the pathological glial fibrillary acidic protein (GFAP) Topo IIα and O⁶-methylguanine-DNA methyltransferase (MGMT) in astrocytomas using magnetic resonance (MR) diffusion kurtosis imaging (DKI) to evaluate the biological characteristics of astrocytomas. MATERIAL AND METHODS Sixty-six patients with pathologically proven astrocytomas were enrolled in this study. All patients underwent conventional MRI head scanning, DKI scanning, and enhanced scanning under the same conditions. Spearman's rank correlation analysis and Bonferroni correction were used to compare the values of DKI and the expression levels of GFAP, Topo IIα, and MGMT between the 2 groups. RESULTS Mean kurtosis (MK) values were negatively correlated with the expression of GFAP (r=-0.836; P=0.03). However, these were positively correlated with the expression of Topo IIα (r=0.896; P=0.01). Moreover, fractional anisotropy (FA) values were not correlated with the expression of GFAP (r=0.366; P=0.05), Topo IIα (r=-0.562; P=0.05), or MGMT (r=-0.153; P=0.10). CONCLUSIONS MK was significantly associated with the expression of GFAP and Topo IIα. To a certain extent, applying DKI may show the biological behavior of tumor cell differentiation, proliferation activity, invasion, and metastasis, and guide individual treatment.

• Authors: Xiao-Chun Wang, Ying Lei, Le Wang, Yan Tan, Jiang-Bo Qin, Guo-Lin Ma, Hui Zhang
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 24 Pg. 8822-8830 (Dec 06 2018) ISSN: 1643-3750 [Electronic] United States
• PMID: 30520434 (Publication Type: Journal Article)

Chemical References

• GFAP protein, human
• Glial Fibrillary Acidic Protein
• Poly-ADP-Ribose Binding Proteins
• Tumor Suppressor Proteins
• DNA Modification Methylases
• MGMT protein, human
• DNA Topoisomerases, Type II
• TOP2A protein, human
• DNA Repair Enzymes

Topics

• Adult
• Aged
• Anisotropy
• Astrocytoma (diagnostic imaging, genetics, metabolism, pathology)
• Brain Neoplasms (diagnostic imaging, genetics, metabolism, pathology)
• DNA Modification Methylases (biosynthesis, genetics, metabolism)
• DNA Repair Enzymes (biosynthesis, genetics, metabolism)
• DNA Topoisomerases, Type II (biosynthesis, genetics, metabolism)
• Diffusion Magnetic Resonance Imaging (methods)
• Diffusion Tensor Imaging (methods)
• Female
• Glial Fibrillary Acidic Protein (biosynthesis, genetics, metabolism)
• Humans
• Magnetic Resonance Imaging
• Male
• Middle Aged
• Poly-ADP-Ribose Binding Proteins (biosynthesis, genetics, metabolism)
• Tumor Suppressor Proteins (biosynthesis, genetics, metabolism)