Nonclustered
protocadherins (PCDH) family is a group of cell-
cell adhesion molecules. We have found differentially methylated genes in the nonclustered PCDHs family associated with Helicobacter pylori (H. pylori)
infection in prior genome-wide methylation analysis. To further investigate the methylation and expression of nonclustered PCDHs encoding genes in H. pylori--related gastric
carcinogenesis process, four candidate genes including PCDH7, PCDH10, PCDH17, and PCDH20 were selected, which were reported to be
tumor suppressors for digestive
cancers. A total of 747 participants with a spectrum of gastric lesions were enrolled from a high-risk population of
gastric cancer. Promoter methylation levels of four genes were significantly higher in H. pylori-positive subjects than the negative group (all P < 0.001). Elevated methylation levels of PCDH10 and PCDH17 were observed with the increasing severity of gastric lesions (both P trend < 0.001). In the
protein expression analysis, PCDH17 expression was inversely associated with gastric lesions; the OR [95% confidence interval (CI)] was 0.49 (0.26-0.95) for chronic
atrophic gastritis (CAG), 0.31 (0.15-0.63) for intestinal
metaplasia, and 0.38 (0.19-0.75) for indefinite dysplasia and dysplasia, compared with superficial
gastritis. In addition, PCDH10 expression was significantly lower in CAG (OR, 0.40; 95% CI, 0.24-0.68). The inverse association between methylation and
protein expression of PCDH10 and PCDH17 was further supported when we explored the methylation and
mRNA expression in The
Cancer Genome Atlas database (all P < 0.001). Our study found elevated promoter methylation and decreased expression of PCDH10 and PCDH17 in advanced gastric lesions, suggesting that elevated PCDH10 and PCDH17 methylation may be an early event in gastric
carcinogenesis.
Cancer Prev Res; 11(11); 717-26. ©2018 AACR.