Quality evaluation for Radix Astragali based on fingerprint, indicative components selection and QAMS.

Abstract

Radix Astragali (RA) is one of the most widely used Chinese herbs prescribed in many Chinese formulas to reinforce 'Qi' and treat vital energy deficiency. This study combined fingerprinting with quantitative analysis multi-components by a single marker (QAMS) to improve the quality control standard for RA on the basis of existing quality control methods of traditional Chinese medicinal materials. UPLC-ESI-TOF-MS technique was used to evaluate the quality of RA by fingerprinting and QAMS. Using the anti-inflammatory, anti-oxidation and anti-anoxic activities to screen characteristic components of RA, the calycosin-7-O-β-d-glucoside (CG), ononin, astragaloside IV, astragaloside II, calycosin and astrageloside I significantly inhibited ear edema in mice, the calycosin and CG had good antioxidant activity and the astragaloside I had a significant anti-hypoxia activity. Astragaloside I, astragaloside II, astragaloside IV, ononin, calycosin and CG had significant pharmacological actions. These components were comprehensively used as the indicative components for the quality control of RA. Astragaloside I was used as the internal standard of the relative correction factors of CG (13.45), ononin (0.51), calycosin (12.08), astragaloside IV (0.73) and astragaloside II (0.81). Astragaloside I and CG were used as internal standards of the relative correction factors of the flavonoids and saponins of ononin (1.11), calycosin (0.04), astragaloside IV (0.73) and astragaloside II (0.81). The study combined fingerprinting with QAMS to improve the quality control standard for RA.

• Authors: Jun Huang, Lei Yin, Lin Dong, Hongfeng Quan, Rong Chen, Shiyao Hua, Jiahua Ma, Dongyan Guo, Xueyan Fu
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 32 Issue 11 Pg. e4343 (Nov 2018) ISSN: 1099-0801 [Electronic] England
• PMID: 30003570 (Publication Type: Journal Article)
• Copyright: © 2018 John Wiley & Sons, Ltd.

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Xylenes
• Huang Qi

Topics

• Animals
• Antioxidants (analysis, chemistry)
• Astragalus propinquus
• Chromatography, High Pressure Liquid (methods)
• Disease Models, Animal
• Drugs, Chinese Herbal (analysis, chemistry)
• Edema (chemically induced)
• Inflammation (chemically induced)
• Linear Models
• Male
• Mice
• Reproducibility of Results
• Spectrometry, Mass, Electrospray Ionization (methods)
• Xylenes (toxicity)