Abstract | OBJECTIVE: Immune cells from patients with rheumatoid arthritis (RA) express more enolase-1 (ENO1) on their surface than those from healthy subjects, and they elicit an enhanced inflammatory response. This study is aimed to identify the ligands of ENO1 that could promote inflammatory loops in vitro and enhance the arthritis severity in vivo. METHODS: ENO1-binding proteins in RA synovial fluid were identified by mass spectromety, and affinity to ENO1 was evaluated by means of a ligand blotting and binding assay, surface plasmon resonance and confocal microscopy. Proinflammatory response by the interaction between ENO1 and apolipoprotein B ( apoB) was tested in vitro and in vivo using peripheral blood mononuclear cells and a K/BxN serum transfer arthritis model and low-density lipoproteins receptor (LDLR) knockout mice. RESULTS: CONCLUSIONS: A key component of atherogenic lipids, apoB, aggravated arthritis by potentiating the inflammatory response via its interaction with ENO1 expressed on the surface of immune cells. This suggests a novel mechanism by which lipid metabolism regulates chronic inflammation in RA.
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Authors | Joo Youn Lee, Min Jueng Kang, Ji Yong Choi, Ji Soo Park, Jin Kyun Park, Eun Young Lee, Eun Bong Lee, Thomas Pap, Eugene C Yi, Yeong Wook Song |
Journal | Annals of the rheumatic diseases
(Ann Rheum Dis)
Vol. 77
Issue 10
Pg. 1480-1489
(10 2018)
ISSN: 1468-2060 [Electronic] England |
PMID | 29997113
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © Author(s) (or their employer(s)) 2018. No commercial re-use. See rights and permissions. Published by BMJ. |
Chemical References |
- Apolipoproteins B
- Biomarkers, Tumor
- Cytokines
- DNA-Binding Proteins
- NF-kappa B
- TNF protein, human
- Tumor Necrosis Factor-alpha
- Tumor Suppressor Proteins
- ENO1 protein, human
- Phosphopyruvate Hydratase
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Topics |
- Apolipoproteins B
(metabolism)
- Arthritis, Rheumatoid
(metabolism)
- Biomarkers, Tumor
(metabolism)
- Cytokines
(biosynthesis)
- DNA-Binding Proteins
(metabolism)
- Humans
- Inflammation
- Leukocytes, Mononuclear
(metabolism)
- NF-kappa B
(metabolism)
- Osteoarthritis
(metabolism)
- Phosphopyruvate Hydratase
(metabolism)
- Signal Transduction
(physiology)
- Synovial Fluid
(metabolism)
- Tumor Necrosis Factor-alpha
(biosynthesis)
- Tumor Suppressor Proteins
(metabolism)
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