Prevention of Fatal C3 Glomerulopathy by Recombinant Complement Receptor of the Ig Superfamily.

Abstract

Background C3 glomerulopathy (C3G) is a life-threatening kidney disease caused by dysregulation of the alternative pathway of complement (AP) activation. No approved specific therapy is available for C3G, although an anti-C5 mAb has been used off-label in some patients with C3G, with mixed results. Thus, there is an unmet medical need to develop other inhibitors of complement for C3G.Methods We used a murine model of lethal C3G to test the potential efficacy of an Fc fusion protein of complement receptor of the Ig superfamily (CRIg-Fc) in the treatment of C3G. CRIg-Fc binds C3b and inhibits C3 and C5 convertases of the AP. Mice with mutations in the factor H and properdin genes (FHm/mP-/-) develop early-onset C3G, with AP consumption, high proteinuria, and lethal crescentic GN.Results Treatment of FHm/mP-/- mice with CRIg-Fc, but not a control IgG, inhibited AP activation and diminished the consumption of plasma C3, factor B, and C5. CRIg-Fc-treated FHm/mP-/- mice also had significantly improved survival and reduced proteinuria, hematuria, BUN, glomerular C3 fragment, C9 and fibrin deposition, and GN pathology scores.Conclusions Therapeutics developed on the basis of the mechanism of action of soluble CRIg may be effective for the treatment of C3G and should be explored clinically.

• Authors: Xiaoxu Wang, Menno Van Lookeren Campagne, Kenneth J Katschke Jr, Damodar Gullipalli, Takashi Miwa, Yoshiyasu Ueda, Yuan Wang, Matthew Palmer, Guolan Xing, Wen-Chao Song
• Journal: Journal of the American Society of Nephrology : JASN (J Am Soc Nephrol) Vol. 29 Issue 8 Pg. 2053-2059 (08 2018) ISSN: 1533-3450 [Electronic] United States
• PMID: 29895552 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 by the American Society of Nephrology.

Chemical References

• Complement C3
• Guanine Nucleotide-Releasing Factor 2
• Receptors, Complement
• VSIG4 protein, mouse
• Complement Factor B

Topics

• Analysis of Variance
• Animals
• Biopsy, Needle
• Blotting, Western
• Complement Activation
• Complement C3 (antagonists & inhibitors, genetics)
• Complement Factor B (immunology, metabolism)
• Disease Models, Animal
• Glomerulonephritis, IGA (genetics, pathology, prevention & control)
• Guanine Nucleotide-Releasing Factor 2 (genetics)
• Immunohistochemistry
• Kidney Function Tests
• Mice
• Mice, Inbred C57BL
• Random Allocation
• Receptors, Complement (genetics, metabolism)
• Survival Rate