Abstract | OBJECTIVE: MATERIALS/METHODS: We cultured primary tracheal epithelial cells in monolayers from both wild-type (WT) and dimethylarginine dimethylaminohydrolase overexpressing C57Bl/6 (DDAH1 transgenic) mice and measured wound repair using the electric cell impedance sensing system. RESULTS:
Wound closure in epithelial cells from WT mice occurred within 24 h in vitro. In contrast, treatment of the WT cell monolayers with 5% hog dust extract prevented significant NO-stimulated wound closure. In cells from DDAH1 transgenic mice, control wounds were repaired up to 8 h earlier than seen in WT mice. A significant enhancement of wound repair was observed in DDAH cells compared to WT cells treated with hog dust extract for 24 h. Likewise, cells from DDAH1 transgenic mice demonstrated increased NO and PKA activity and decreased hog dust extract-stimulated PKC. DISCUSSION/CONCLUSION:
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Authors | Deepak Chandra, Jill A Poole, Kristina L Bailey, Elizabeth Staab, Jenea M Sweeter, Jane M DeVasure, Debra J Romberger, Todd A Wyatt |
Journal | Inhalation toxicology
(Inhal Toxicol)
Vol. 30
Issue 3
Pg. 133-139
(02 2018)
ISSN: 1091-7691 [Electronic] England |
PMID | 29793367
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Dust
- Nitric Oxide
- N,N-dimethylarginine
- Arginine
- Cyclic AMP-Dependent Protein Kinases
- Protein Kinase C
- Amidohydrolases
- dimethylargininase
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Topics |
- Amidohydrolases
(genetics)
- Animal Husbandry
- Animals
- Arginine
(analogs & derivatives, metabolism)
- Cells, Cultured
- Cyclic AMP-Dependent Protein Kinases
(metabolism)
- Dust
- Epithelial Cells
(physiology)
- Mice, Inbred C57BL
- Mice, Transgenic
- Nitric Oxide
(metabolism)
- Protein Kinase C
(metabolism)
- Trachea
(cytology)
- Wound Healing
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