Abstract | INTRODUCTION: MATERIALS AND METHODS: RESULTS:
Insulin could be fleetly and efficiently loaded via the nanocarrier PEG-CMCS at pH =6, showing efficient loading and stable release. In addition, insulin/PEG-CMCS showed significant hypoglycemic activity in diabetic rats. On the other hand, ischemia/reperfusion obviously augmented the contents of creatine kinase (CK), lactic dehydrogenase (LDH), putrescine (Pu), myocardial infarct size, and NF-κB and spermidine/ spermine N'- acetyltransferase (SSAT) expressions and decreased the levels of spermine (Sp), polyamine pools (PAs), heart rate (HR), coronary blood flow (CF), left ventricular developed pressure (LVDP), and ODC expression, compared with Sham. Administration of insulin and insulin/PEG-CMCS both reduced the contents of CK, LDH, Pu, myocardial infarct size, and NF-κB and SSAT expressions and increased the levels of Sp, PAs, HR, CF, LVDP, and ODC expression, while insulin/PEG-CMCS significantly indicated the protective results, and DFMO- EGBG showed the opposite effects. CONCLUSION: The research showed that insulin/PEG-CMCS could play a protective effect on HR/RI in diabetic rats via its antioxidative, antiapoptotic, and anti-inflammatory roles and modulating ODC/ polyamine systems.
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Authors | Fei Tong, Suhuan Liu, Bing Yan, Xuejun Li, Shiwei Ruan, Shuyu Yang |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 13
Pg. 2507-2520
( 2018)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 29719397
(Publication Type: Journal Article)
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Chemical References |
- Cardiotonic Agents
- Drug Carriers
- Insulin
- Polyamines
- insulin, polyethylene glycol(B1)-
- Polyethylene Glycols
- Chitosan
- Ornithine Decarboxylase
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Topics |
- Animals
- Apoptosis
- Cardiotonic Agents
(administration & dosage, pharmacology)
- Chitosan
(analogs & derivatives, chemistry)
- Coronary Vessels
(physiopathology)
- Diabetes Mellitus, Experimental
(drug therapy, physiopathology)
- Drug Carriers
(administration & dosage, pharmacology)
- Insulin
(administration & dosage, analogs & derivatives, pharmacology)
- Ischemic Preconditioning, Myocardial
(methods)
- Male
- Myocardial Infarction
(pathology)
- Myocardial Reperfusion Injury
(drug therapy)
- Nanoparticles
(administration & dosage, chemistry)
- Ornithine Decarboxylase
(metabolism)
- Polyamines
(metabolism)
- Polyethylene Glycols
(administration & dosage, chemistry, pharmacology)
- Rats, Sprague-Dawley
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