Kaempferol modulates Angiopoietin-like protein 2 expression to lessen the mastitis in mice.

Abstract

BACKGROUND:

Mastitis is inflammation of a breast (or udder). Angiopoietin-like protein 2 (ANGPTL2) has been found as a key inflammatory mediator in mastitis. Purpose of this research was to investigate the mechanisms about repressing effect of kaempferol on mastitis.

METHODS:

Forty mice were randomly divided into 4 groups (n=10): C57BL/6J control mice, untreated murine mastitis, 10mg/kg kaempferol treated murine mastitis (ip), and 30mg/kg kaempferol treated murine mastitis (ip). Primary cultured mouse mammary epithelial cells (MMEC) were indiscriminately divided into seven groups including control group, 10mmol/L vehicle of kaempferol group, 10μmol/L kaempferol treated group, 20μg/mL LPS treated group, 1μmol/L kaempferol plus LPS treated group, 3μmol/L kaempferol plus LPS treated group, and 10μmol/L kaempferol plus LPS treated group.

RESULTS:

In murine mastitis, kaempferol (10 or 30mg/kg) treatment prevented mastitis development, decreased myeloperoxidase (MPO) production, interleukin (IL)-6 level, tumour necrosis factor-α (TNF-α) concentration, and ANGPTL2 expression. In MMEC, kaempferol (1, 3 or 10μM) reduced MPO production, TNF-α concentration, IL-6 level, and ANGPTL2 expression.

CONCLUSIONS:

The results in present study show that kaempferol modulates the expression of ANGPTL2 to lessen the mastitis in mice.

• Authors: Hong-Bo Xiao, Guo-Guang Sui, Xiang-Yang Lu, Zhi-Liang Sun
• Journal: Pharmacological reports : PR (Pharmacol Rep) Vol. 70 Issue 3 Pg. 439-445 (Jun 2018) ISSN: 2299-5684 [Electronic] Switzerland
• PMID: 29627690 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.

Chemical References

• Angiopoietin-Like Protein 2
• Angiopoietin-like Proteins
• Angptl2 protein, mouse
• Interleukin-6
• Kaempferols
• Tumor Necrosis Factor-alpha
• kaempferol
• Peroxidase

Topics

• Angiopoietin-Like Protein 2
• Angiopoietin-like Proteins (metabolism)
• Animals
• Epithelial Cells (drug effects, metabolism)
• Female
• Inflammation (drug therapy, metabolism)
• Interleukin-6 (metabolism)
• Kaempferols (pharmacology)
• Mastitis (drug therapy, metabolism)
• Mice
• Mice, Inbred C57BL
• Peroxidase (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)