In this study, we investigated the effects of dietary myo-
inositol on the intestinal immune barrier function and related signaling pathway in young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.33 ± 0.84 g) were fed six diets containing graded levels of myo-
inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila. The results indicated that compared with the optimal dietary myo-
inositol level, myo-
inositol deficiency (27.0 mg/kg diet): (1) decreased
lysozyme (LZ) and
acid phosphatase (ACP) activities, as well as
complement 3 (C3), C4 and
immunoglobulin M (
IgM) contents in the proximal intestine (PI), middle intestine (MI) and distal intestine (DI) of young grass carp (P < 0.05). (2) down-regulated the
mRNA levels of anti-microbial substance:
liver expressed antimicrobial peptide (LEAP) 2A, LEAP-2B,
hepcidin, β-defensin-1 and mucin2 in the PI, MI and DI of young grass carp (P < 0.05). (3) up-regulated pro-inflammatory
cytokines [IL-1β (not in DI), TNF-α and IL-8],
nuclear factor kappa B P65 (not NF-κB P52), c-Rel, IκB kinaseα (IKKα), IKKβ and IKKγ
mRNA levels in the PI, MI and DI of young grass carp (P < 0.05); and down-regulated pro-inflammatory
cytokines IL-15 (not in DI) and inhibitor of κBα (IκBα)
mRNA levels (P < 0.05). (4) down-regulated the
mRNA levels of anti-inflammatory
cytokines [IL-10 (not in DI),
IL-11, IL-4/13B (not IL-4/13A), TGF-β1 and TGF-β2], target of
rapamycin (TOR), eIF4E-binding
proteins 1 (4E-BP1) and
ribosomal protein S6 kinase 1 (S6k1) in the PI, MI and DI of young grass carp (P < 0.05). All data indicated that myo-
inositol deficiency could decrease fish intestine immunity and cause
inflammation under
infection of A. hydrophila. Finally, the optimal dietary myo-
inositol levels for the ACP and LZ activities in the DI were estimated to be 415.1 and 296.9 mg/kg diet, respectively.