Modulation of pro-apoptotic effects and mitochondrial potential on B16F10 cells by DODAC/PHO-S liposomes.

Abstract

OBJECTIVE:

We aimed to evaluate the potential of DODAC/PHO-S liposomes on the modulation of the expression of pro-apoptotic proteins, loss of lysosomal integrity and the mitochondrial electrical potential, compared with phosphoethanolamine.

RESULTS:

The results of this study demonstrate that DODAC/PHO-S liposomes have exhibited broad cytotoxic potential in B16F10 murine melanoma cells, with significantly greater proportions than treatment with PHO-S. The treatment with the DODAC/PHO-S 2.0 mM liposomal formulation was more efficient in decreasing mitochondrial electrical potential at the same concentrations and treatment time than PHO-S The liposomal formulation DODAC/PHO-S (2.0 mM) was more efficient to promote morphological changes in the cells, without presenting intact lysosomes, at the same time of treatment and concentration as PHO-S Our results demonstrated that the liposomal formulation increased DR4 receptor expression and activated caspases 8 and 3, resulting in the release of cytochrome c in B16F10 tumour cells, when compared to treatment with PHO-S The data obtained prove that the use of DODAC as carrier can maximize the cytotoxic effects of PHO-S This was demonstrated by the translocation of cytochrome c to the cytoplasm and activation of caspase-3 and 8, decreasing the mitochondrial electrical potential and generating morphological changes, in B16F10 cells.

• Authors: Arthur Cássio de Lima Luna, José Roberto de Assis Santos Filho, Henrique Hesse, Salvador Claro Neto, Gilberto Orivaldo Chierice, Durvanei Augusto Maria
• Journal: BMC research notes (BMC Res Notes) Vol. 11 Issue 1 Pg. 126 (Feb 14 2018) ISSN: 1756-0500 [Electronic] England
• PMID: 29444697 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Adjuvants, Immunologic
• Cytotoxins
• Ethanolamines
• Liposomes
• Quaternary Ammonium Compounds
• dimethyldioctadecylammonium
• phosphorylethanolamine

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cytotoxins (pharmacology)
• Ethanolamines (pharmacology)
• Liposomes (pharmacology)
• Melanoma, Experimental (drug therapy)
• Mice
• Mitochondria (drug effects)
• Nanotechnology
• Quaternary Ammonium Compounds (pharmacology)