Proteomics analysis identified peroxiredoxin 2 involved in early-phase left ventricular impairment in hamsters with cardiomyopathy.

Abstract

Given the hypothesis that inflammation plays a critical role in the progression of cardiovascular diseases, the aim of the present study was to identify new diagnostic and prognostic biomarkers of myocardial proteins involved in early-phase cardiac impairment, using proteomics analysis. Using the two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) combined with MALDI-TOF/TOF tandem mass spectrometry, we compared differences in the expression of proteins in the whole left ventricles between control hamsters, dilated cardiomyopathic hamsters (TO-2), and hypertrophy cardiomyopathic hamsters (Bio14.6) at 6 weeks of age (n = 6, each group). Proteomic analysis identified 10 protein spots with significant alterations, with 7 up-regulated and 3 down-regulated proteins in the left ventricles of both TO-2 and Bio 14.6 hamsters, compared with control hamsters. Of the total alterations, peroxiredoxin 2 (PRDX2) showed significant upregulation in the left ventricles of TO-2 and Bio 14.6 hamsters. Our data suggest that PRDX2, a redox regulating molecule, is involved in early-phase left ventricular impairment in hamsters with cardiomyopathy.

• Authors: Kentaro Kuzuya, Sahoko Ichihara, Yuka Suzuki, Chisa Inoue, Gaku Ichihara, Syota Kurimoto, Shinji Oikawa
• Journal: PloS one (PLoS One) Vol. 13 Issue 2 Pg. e0192624 ( 2018) ISSN: 1932-6203 [Electronic] United States
• PMID: 29438398 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peroxiredoxins

Topics

• Animals
• Body Weight
• Cardiomyopathies (metabolism, physiopathology)
• Cricetinae
• Electrophoresis, Gel, Two-Dimensional
• Heart Ventricles (metabolism, physiopathology)
• Male
• Mesocricetus
• Peroxiredoxins (metabolism)
• Proteomics
• Reverse Transcriptase Polymerase Chain Reaction