Abstract | BACKGROUND & AIM: METHODS: Two different DNA-encoded viruses, vaccinia virus (VACV) and murine cytomegalovirus (MCMV), were studied. In vivo imaging was applied to visualize local IFN-β induction and IFN-I receptor (IFNAR) triggering in VACV-infected reporter mice. Furthermore, mice with a cell type-selective IFNAR ablation were analyzed to dissect the role of IFNAR signaling in myeloid cells and hepatocytes. Experiments with Cx3cr1+/gfp mice revealed the origin of reconstituted KC. Finally, mixed bone marrow chimeric mice were studied to specifically analyze the effect of IFNAR triggering on liver infiltrating monocytes. RESULTS: VACV infection induced local IFN-β responses, which lead to IFNAR signaling primarily within the liver. IFNAR triggering was needed to control the infection and prevent fulminant hepatitis. The severity of liver inflammation was independent of IFNAR triggering of hepatocytes, whereas IFNAR triggering of myeloid cells protected from excessive inflammation. Upon VACV or MCMV infection KC disappeared, whereas infiltrating monocytes differentiated to KC afterwards. During IFNAR triggering such replenished monocyte-derived KC comprised more IFNAR-deficient than -competent cells in mixed bone marrow chimeric mice, whereas after the decline of IFNAR triggering both subsets showed an even distribution. CONCLUSION: Upon VACV infection IFNAR triggering of myeloid cells, but not of hepatocytes, critically modulates acute viral hepatitis. During infection with DNA-encoded viruses IFNAR triggering of liver-infiltrating blood monocytes delays the development of monocyte-derived KC, pointing towards new therapeutic strategies for acute viral hepatitis. LAY SUMMARY:
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Authors | Katharina Borst, Theresa Frenz, Julia Spanier, Pia-Katharina Tegtmeyer, Chintan Chhatbar, Jennifer Skerra, Luca Ghita, Sukumar Namineni, Stefan Lienenklaus, Mario Köster, Mathias Heikenwaelder, Gerd Sutter, Ulrich Kalinke |
Journal | Journal of hepatology
(J Hepatol)
Vol. 68
Issue 4
Pg. 682-690
(04 2018)
ISSN: 1600-0641 [Electronic] Netherlands |
PMID | 29274730
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2017 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Receptor, Interferon alpha-beta
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Topics |
- Acute Disease
- Animals
- Hepatitis, Viral, Animal
(etiology, physiopathology)
- Kupffer Cells
(physiology)
- Mice
- Mice, Inbred C57BL
- Receptor, Interferon alpha-beta
(physiology)
- Signal Transduction
(physiology)
- Vaccinia
(physiopathology)
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