Expression of ESR1, PGR, HER2 and Ki67 is important for risk stratification and
therapy in
breast cancer.
Hormone receptor expression can also be found in MIBC, reflecting
luminal and basal subtypes of
breast cancer. Thus the purpose was to investigate on the
mRNA expression of the aforementioned markers and their prognostic value in pT1
bladder cancer. Retrospective analysis of clinical data and
Formalin-Fixed
Paraffin-Embedded tissues (FFPE) of patients with stage pT1
NMIBC who underwent transurethral resection of the bladder was performed.
mRNA expression was measured by single step RT-qPCR. Relative gene expression was determined by normalization to two housekeeping genes (CALM2, B2M) using the 40-ΔΔCT method. Correlation of
mRNA expression with outcome was assessed using Kaplan-Meier analysis and multivariate Cox regression analysis. From overall 302 patients, 255 samples could be analyzed with valid measurements. Subtype distribution was
Luminal-A in 11.4%,
Luminal-B in 38.8%, triple negative in 36.9% and ERBB2 in 12.9%, respectively. Kaplan-Meier analysis revealed molecular subtyping being statistical significant for RFS (p=0.0408) and PFS (p=0.0039).
Luminal-A patients did have the best RFS and PFS. Multivariate analysis revealed molecular subtyping to be significant for PFS (L-R Chi2 of 11.89, p=0.0078). Elevated expression of HER2 was statistically significant for PFS (p=0.0025) and discriminated among G3
tumors a high risk group (60% PFS) from a low risk risk group (90% PFS) after 5 year follow-up (p<0.001). Expression of ESR1, PGR and HER2 has predictive value in stage pT1
NMIBC and reveals potential therapeutic targets.