Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice.

Abstract

Avian-like H1N1 swine influenza viruses are prevalent in pigs and have occasionally crossed the species barrier and infected humans, which highlights the importance of preventing swine influenza. Human adenovirus serotype 5 (Ad5) has been tested in human influenza vaccine clinical trials and has exhibited a reliable safety profile. Here, we generated a replication-defective, recombinant adenovirus (designated as rAd5-avH1HA) expressing the hemagglutinin gene of an avian-like H1N1 virus (A/swine/Zhejiang/199/2013, ZJ/199/13). Using a BALB/c mouse model, we showed that a two-dose intramuscular administration of recombinant rAd5-avH1HA induced high levels of hemagglutination inhibition antibodies and prevented homologous and heterologous H1N1 virus-induced weight loss, as well as viral replication in the nasal turbinates and lungs of mice. Furthermore, a prime-boost immunization strategy trial with a recombinant plasmid (designated as pCAGGS-HA) followed by rAd5-avH1HA vaccine provided effective protection against homologous and heterologous H1N1 virus infection in mice. These results indicate that rAd5-avH1HA is an efficacious genetically engineered vaccine candidate against H1N1 swine influenza. Future studies should examine its immune efficacy in pigs.

• Authors: Yunpu Wu, Dawei Yang, Bangfeng Xu, Wenhua Liang, Jinyu Sui, Yan Chen, Huanliang Yang, Hualan Chen, Ping Wei, Chuanling Qiao
• Journal: Antiviral research (Antiviral Res) Vol. 147 Pg. 29-36 (Nov 2017) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 28941982 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Hemagglutinin Glycoproteins, Influenza Virus
• Influenza Vaccines
• Vaccines, Synthetic

Topics

• Adenoviridae (genetics)
• Animals
• Antibodies, Viral (blood)
• Antigens, Viral (immunology)
• Cross Protection
• Hemagglutination Inhibition Tests
• Hemagglutinin Glycoproteins, Influenza Virus (genetics, immunology)
• Influenza A Virus, H1N1 Subtype (genetics, immunology)
• Influenza Vaccines (administration & dosage, genetics, immunology, standards)
• Lung (virology)
• Mice
• Mice, Inbred BALB C
• Orthomyxoviridae Infections (prevention & control)
• Swine
• Swine Diseases (prevention & control)
• Turbinates (virology)
• Vaccines, Synthetic (administration & dosage, genetics, immunology)
• Virus Shedding